Beyond the Monolayer: Leveraging 3D Culture Systems to Decode Mechanisms of Drug Resistance and Therapeutic Vulnerabilities in Colorectal Cancer. (PubMed, Cancer Med) - Oct 1, 2026 - "Building on this foundation, we highlight recent technical innovations and discuss how integrating morphometric, metabolic, and trajectory-level readouts can move CRC drug discovery "beyond live/dead." Rather than propose a single best model, we outline practical guidelines for matching 3D platform choice (cell lines vs. PDO/CTOS, collagen vs. Matrigel, static vs. live-imaging HTS) to mechanistic questions and therapeutic goals in CRC. We propose an integrated roadmap-matrix-embedded 3D cultures, high-content morphological endpoints, trajectory analytics, and rigorous HTS validation-to decode resistance mechanisms and prioritize clinically actionable combinations in CRC."
Journal • Review • Colorectal Cancer • Oncology • Solid Tumor
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Bhuminder Singh; Sarah J Harmych
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