Analysis of motor-based transport in primary cilia by dynamic mode decomposition of live-cell imaging data. (PubMed, J Cell Sci) - Sep 16, 2026 - "However, when retrograde dynein-2 function is inhibited by Ciliobrevin D or Tanespimycin, both anterograde and retrograde IFT velocities decrease in parental cells, as expected, but remain unchanged in KIF13B mutant cells. Structured illumination, confocal, and STED microscopy further show that KIF13B localizes to the ciliary membrane and concentrates at the periciliary membrane region and the centriolar subdistal appendages, below the distal appendage marker FBF1. Our improved kymograph approach provides new insight into KIF13B ciliary function and simplifies the quantitative analysis of ciliary protein transport."
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Daniel Wüstner; Fabiola Campestre; Line Lauritsen; Lotte B Pedersen
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