Resolving conformational polymorphism in disulfide-rich peptide drugs. (PubMed, Front Chem) - Sep 2, 2026 - "Here, cyclic ion mobility-mass spectrometry (cIM-MS), combined with accelerated thermal stress testing, was applied to a manufacturing batch of ziconotide and linaclotide under native and thermally stressed conditions. The high-resolution separation achieved by cIM-MS enabled detailed mapping of coexisting conformers and their stress-induced transitions. These results demonstrate that cIM-MS provides a spatially resolved analytical platform for interrogating higher-order structural heterogeneity in disulfide-rich peptide therapeutics, extending the capabilities of conventional mass spectrometry and offering a generalizable approach for structural characterization and quality assessment."
Journal
|
|
Haiwei Huang; Mingzhe Xu; Shaozhou Zhu; Yifeng Ge
|