Development and Validation of an Optimized HPLC-DAD Method for Simultaneous Quantification of 14 Bioactive Compounds in Lonicerae. (PubMed, Planta Med) - Sep 24, 2026 - "The validated method exhibited excellent linearity (R² > 0.9990), sensitivity (LOQ ≤ 0.40 μg/mL, LOD ≤ 0.12 μg/mL), precision (intra- and inter-day RSD ≤ 3.59%), and recovery (97.03-102.62%), with satisfactory stability over 24 h. When applied to Lonicerae Flos samples from three geographical regions, the method revealed significant variations in chlorogenic acid, neochlorogenic acid, and 3,4-dicaffeoylquinic acid, highlighting its utility for geographical origin tracing and regional quality assessment...Compared with existing methods, the proposed approach demonstrates superior performance with wider analyte coverage, improved separation efficiency, and simplified sample pretreatment. Accordingly, this work provides a practical tool to address the key challenges in the quality control of this medicinal plant, particularly the complex and variable profiles of bioactive constituents, thus supporting the standardization and quality evaluation of..."
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Hongjing Zhang; Jinghua Li; Xiaoyuan Peng; Xizhe Sun; Ye Li
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