Renoprotective effects of C-peptide in the Dahl salt sensitive rat (Am J Physiol Renal Physiol) - Jul 20, 2012 - Urine albumin (UAE) & protein (UPE) increased by 74% & 92%, respectively, from baseline & time of sacrifice in HS2+veh rats; HS2+Cpep reduced glomerular permeability, tubulointerstitial fibrosis, renal inflammation, TGF-β & podocin protein expression while HS4+Cpep had no effect; C-peptide is renoprotective in non-diabetic experimental models with mild to moderate renal injury
Preclinical-animal • Renal Disease
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Am J Physiol Renal Physiol. 2012 Jul 18. [Epub ahead of print]
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Abstract
Previous studies have demonstrated that renoprotective effects of C-peptide in experimental models of diabetes-induced renal disease may be mediated via lowering blood glucose. The present study examined the renoprotective effects of C-peptide in a model of non-diabetic renal disease, the Dahl salt-sensitive (SS/jr) rat. SS/jr rats were placed on a 2% NaCl diet for 2 weeks (HS2, resulting in mild to moderate renal injury) or 4 weeks (HS4, resulting in advanced renal injury) then received either vehicle (veh) or C-peptide (Cpep) for additional 4 weeks. Urine albumin (UAE) and protein (UPE) excretion rates were measured at baseline (i.e. prior to initiation of veh or Cpep treatment) and 4 weeks later (i.e. at the time of sacrifice). Glomerular permeability, indices of glomerulosclerosis and tubulointerstitial fibrosis, presence of inflammatory cells and protein expression of transforming growth factor beta (TGF-β) and podocin were measured at the time of sacrifice. In HS2+veh rats, UAE and UPE increased by 74% and 92%, respectively, from baseline and the time of sacrifice. While HS2+Cpep attenuated this increase in UAE and UPE, HS4+Cpep had no effect on these parameters. Similarly, HS2+Cpep reduced glomerular permeability, tubulointerstitial fibrosis, renal inflammation, TGF-β and podocin protein expression, while HS4+Cpep had no effect. These studies indicate that C-peptide is renoprotective in non-diabetic experimental models with mild to moderate renal injury.
PMID: 22811482
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