allogeneic human dental pulp stem cells / Utooth Biological Technology, Wuhan University, Beijing SH Bio-Tech Corporation - LARVOL DELTA

Clinical-grade human dental pulp stem cells improve adult hippocampal neural regeneration and cognitive deficits in Alzheimer's disease. (PubMed, Theranostics) - " These findings highlight the multifunctional potential of hDPSCs in AD treatment, which enhances cognition through alleviating neuropathology and providing neural regenerative driving force. Understanding these multiplicity effects is critical to advancing the clinical translation of stem cell-based therapies for AD."

Journal • Alzheimer's Disease • CNS Disorders • Cognitive Disorders • Transplantation

Peripheral Lysosomal Positioning in Inflamed Odontoblasts Facilitates Mineralization. (PubMed, J Endod) - "Inflammatory stimuli prompted a relocation of lysosomes in odontoblasts, redistributing them from perinuclear location towards the cell periphery, which in turn facilitated mineralization, potentially via mTORC1 signaling and lysosomal exocytosis."

Journal • Inflammation • LAMP1 • SNAPIN

Role and Molecular Mechanism of miR-586 in the Differentiation of Dental Pulp Stem Cells into Odontoblast-like Cells. (PubMed, Cell Biochem Biophys) - "In this article, human dental pulp stem cells (hDPSCs) were used as samples, and hDPSCs were co-cultured with endothelial progenitor cells (EPCs)...The number of mineralized nodules was significantly increased (P < 0.05). MiR-586 plays a key regulatory function in DPSCs differentiated into odontoblast-like cells and is associated with specific molecular mechanisms."

Journal • DSPP

Yeast β-glucan-based nanoparticles loading methotrexate promotes osteogenesis of hDPSCs and periodontal bone regeneration under the inflammatory microenvironment. (PubMed, Carbohydr Polym) - "Additionally, cBPM inhibited inflammation and repaired alveolar bone by transforming macrophage phenotype from inflammatory M1 to anti-inflammatory M2. This work provides an alternative strategy for the clinical treatment of periodontitis through BYG-based delivery nanoplatform of anti-inflammatory drugs."

Journal • Dental Disorders • Inflammation • Periodontitis • RUNX2

The Impact of the VEGF/VEGFR2/PI3K/AKT Signaling Axis on the Proliferation and Migration Abilities of Human Dental Pulp Stem Cells. (PubMed, Cell Biochem Biophys) - "The VEGF/VEGFR2 signaling axis regulated the migration ability of HDPSCs through the FAK/PI3K/AKT and P38MAPK pathways. This finding highlighted not only the crucial role of VEGF in injury repair of HDPSCs but also provided important clues for a comprehensive understanding of the potential applications of this signaling axis in dental regenerative medicine."

Journal

Human dental pulp stem cells mitigate the neuropathology and cognitive decline via AKT-GSK3β-Nrf2 pathways in Alzheimer's disease. (PubMed, Int J Oral Sci) - "Mechanistically, antioxidant and neuroprotective effects, as well as cognitive enhancements elicited by hDPSCs, were at least partially mediated by Nrf2 nuclear accumulation and downstream antioxidant enzymes expression through the activation of the AKT-GSK3β-Nrf2 signaling pathway. In conclusion, our findings corroborated the neuroprotective capacity of hDPSCs to reshape the neuropathological microenvironment in both in vitro and in vivo AD models, which may be a tremendous potential therapeutic candidate for Alzheimer's disease."

Journal • Alzheimer's Disease • Bone Marrow Transplantation • CNS Disorders • Transplantation

Human dental pulp stem cells are subjected to metabolic reprogramming and repressed proliferation and migration by the sympathetic nervous system via ADRA1B. (PubMed, J Endod) - "This study demonstrates that the SNS can shift the metabolism of hDPSCs from oxidative phosphorylation to anaerobic glycolysis via ADRA1B and its crosstalk with serine-threonine kinase and p38 mitogen-activated protein kinase signaling pathways, thereby inhibiting the proliferative and migratory abilities of hDPSCs. This metabolic shift may facilitate the maintenance of the quiescent state of hDPSCs."

Journal • ADRA1B

The N6-methyladenosine demethylase FTO is required for odontoblast differentiation in vitro and dentine formation in mice by promoting RUNX2 exon 5 inclusion through RBM4. (PubMed, Int Endod J) - "Thus, within the limitations of this study, the data suggest that FTO promotes odontoblastic differentiation during dentine formation by stabilizing RBM4 mRNA to promote RUNX2 exon 5 inclusion."

Journal • Preclinical • Genetic Disorders • Obesity • FTO • RBM4 • RUNX2

HnRNP A1 Suppresses the Odontogenic Differentiation and the Inclusion of RUNX2 Exon 5 of Dental Mesenchymal Cells. (PubMed, Front Biosci (Landmark Ed)) - "The present study indicated that hnRNP A1 suppressed RUNX2 exon 5 inclusion and reduced the odontogenic differentiation ability of hDPSCs and mDPCs."

Journal • ALPL • RUNX2 • YBX1

GATA4 inhibits odontoblastic differentiation of dental pulp stem cells through targeting IGFBP3. (PubMed, Arch Oral Biol) - "GATA4 inhibited odontoblastic differentiation of HDPSCs via activating the transcriptional activity of IGFBP3, identifying its promising role in regulating HDPSCs odontoblast differentiation and reparative dentinogenesis."

Journal • IGFBP3

Effects of RNA m6A Writer METTL3 and hDPSCs on the peripheral nerve regeneration: in vitro and in vivo study. (PubMed, Neurosci Lett) - "These results demonstrated that RNA m6A participated in the differentiation and proliferation of dental pulp stem cells, and that OE-METTL3 group exhibited the greater ability to improve peripheral nerve regeneration than KD-METTL3 group and hDPSCs group."

Journal • Preclinical • Transplantation • METTL3

Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke. (PubMed, Neural Regen Res) - "We found that anti34a-hDPSCs significantly inhibited apoptosis, reduced cerebral edema and cerebral infarct volume, and improved motor function in mice. This study provides new insights into the molecular mechanism of the cell proliferation and antioxidant capacity of hDPSCs, and suggests a potential gene that can be targeted to improve the survival rate and efficacy of transplanted hDPSCs in brain after ischemic stroke."

Journal • Cardiovascular • Ischemic stroke • Reperfusion Injury • Transplantation • MIR34A • SIRT1

Fabrication of an exosome-loaded thermosensitive chitin-based hydrogel for dental pulp regeneration. (PubMed, J Mater Chem B) - "To address this challenge, exosomes were isolated from human pulp stem cells (hDPSCs) and directly embedded into the HPCH/CW pre-gel to form an exosome-loaded hydrogel (HPCH/CW/Exo)...Meanwhile, in vivo animal experiments revealed the formation of new dental pulp-like tissues in an implanted tooth root model. Therefore, the proposed hydrogel provides a great potential alternative to traditional root canal therapy in dental clinics."

Journal

BACH1 regulates the proliferation and odontoblastic differentiation of human dental pulp stem cells. (PubMed, BMC Oral Health) - "Our findings suggest that BACH1 is an important regulator of the proliferation and odontoblastic differentiation of hDPSCs in vitro. Manipulation of BACH1 expression may provide an opportunity to promote the regenerative capacity of hDPSCs."

Journal • BACH1 • BRIP1 • HMOX1

Single-cell characterization of monolayer cultured human dental pulp stem cells with enhanced differentiation capacity. (PubMed, Int J Oral Sci) - "Taken together, our study for the first time revealed the cellular composition switch of monolayer cultured hDPSCs compared to the freshly isolated hDPSCs. After in vitro expansion, the MCAM(+)JAG(+)PDGFRA(-) subpopulation resembled the most transcriptional characteristics of fresh hDPSCs which may be beneficial for further tissue regeneration applications."

Journal • Dental Disorders • PDGFRA

Safety and Efficacy Study of Allogeneic Human Dental Pulp Mesenchymal Stem Cells to Treat Severe COVID-19 Patients (clinicaltrials.gov) - P1/2; N=20; Recruiting; Sponsor: Renmin Hospital of Wuhan University; Trial completion date: Mar 2021 ➔ Dec 2021; Trial primary completion date: Dec 2020 ➔ Nov 2021

Clinical • Trial completion date • Trial primary completion date • Infectious Disease • Novel Coronavirus Disease • Pneumonia • Respiratory Diseases • CD4 • CD8 • CRP • NCAM1 • TNFA

Safety and Efficacy Study of Allogeneic Human Dental Pulp Mesenchymal Stem Cells to Treat Severe COVID-19 Patients (clinicaltrials.gov) - P1/2; N=20; Recruiting; Sponsor: Renmin Hospital of Wuhan University

Clinical • New P1/2 trial