BMN 053 / BioMarin - LARVOL DELTA

Effect of Different Caenorhabditis elegans U6 Promoters on the Efficiency of CRISPR/Cas9-Mediated Gene Editing (PubMed, Sichuan Da Xue Xue Bao Yi Xue Ban) - "We screened endogenous U6 small nuclear RNA (snRNA) genes of C. elegans from the WormBase database and constructed 14 editing plasmids targeting dpy-10 by replacing the U6 r07e5.16 promoter in the pSX524 plasmid (Peft-3::cas9::tbb-2 terminator::U6 r07e5.16::dpy-10 sgRNA) through molecular cloning...In this study, U6 promoters that significantly improve gene editing efficiency in C. elegans are identified and the critical role of promoter optimization in CRISPR-Cas9 systems is highlighted. These findings provide a valuable foundation for improving genome editing strategies and offer new ideas for optimizing the CRISPR technology applied in nematode research."

Journal

[VIRTUAL] PS524. Contemporary Management of Head and Neck Trauma (ACS-CLINCON 2021) - "This Panel Session emphasizes the surgeon's role within the context of the team approach for treating significant traumatic injuries to the head and neck from gunshot and stab wounds. Vascular and visceral organ repair will be emphasized to provide guidance in the optimal functional repair of the head and neck."