chymotrypsin / Generic mfg. - LARVOL DELTA

Deep Learning-Guided Discovery of Dual Inhibitors of SARS-CoV-2 Entry and 3CL Protease. (PubMed, Molecules) - "Here, we developed a Deep Learning-based Activity Screening Model (DLASM), which integrates graph convolutional network with machine learning to identify SARS-CoV-2 inhibitors, using experimental 3-chymotrypsin-like (3CL) main protease assay data...Together, these findings establish dual-target inhibition as a promising antiviral strategy, offering not only enhanced potency but also reduced risk of resistance. Moreover, our DLASM framework provides a generalizable pipeline for identifying chemically diverse scaffolds and for broader applications beyond SARS-CoV-2."

Journal • Cognitive Disorders • Infectious Disease • Novel Coronavirus Disease • Respiratory Diseases

Beef-Derived Peptides Mediated Desensitization of Bitter Taste Receptor T2R14 Through GPCR Kinase 2. (PubMed, Nutrients) - "Our earlier research showed that peptides from alcalase and chymotrypsin hydrolysates of beef proteins inhibited quinine-dependent calcium release through T2R4, with AGDDAPRAVF and ETSARHL showing the greatest effectiveness...Furthermore, GRK2 knockout in HEK cells stably expressing T2R14 decreases intracellular calcium release, as measured by the area under the curve (AUC), and also delays the fall time (indication of desensitization) of the calcium response when exposed to the T2R14 agonist diphenhydramine (DPH) or beef protein-derived agonist peptide TMTL... Thus, this study showed that beef protein-derived peptides can function as both T2R inhibitors and mediate T2R14 desensitization through GRK2 signaling. These antagonistic food protein-derived peptides inform strategies to enhance nutrition, such as promoting healthier food choices by reducing bitterness and thereby improving the palatability of health-promoting bitter foods, such as fruit and vegetable extracts,..."

Journal

Hypoxia Increases Connexin46 and Connexin43 Levels in KNS-42 Glioblastoma Cells. (PubMed, Int J Mol Sci) - "The cells that survived temozolomide treatment had increased connexin46 levels and decreased connexin43 levels...Exposure to hypoxia for 12 days decreased the chymotrypsin-like proteasomal activity without altering connexin mRNA levels, suggesting that the changes in connexin levels result from reduced protein degradation...The results also imply that changes in the microenvironment of glioblastomas (like hypoxia) can alter proteasomal activity and affect levels and subcellular distribution of connexin46 and connexin43. Finally, our data suggest that proteasomal inhibition may not be a good approach to glioblastoma therapy."

Journal • Brain Cancer • Glioblastoma • Oncology • Solid Tumor • Targeted Protein Degradation

Squaraine dyes for biophotonics: sensing, imaging and theranostics. (PubMed, Chem Soc Rev) - "The sensitivity of squaraine dyes in biomolecular recognition is highlighted for detecting proteins such as bovine serum albumin (BSA) and enzymes like chymotrypsin...This review also highlights the emerging theranostic potential of squaraine dyes specifically for targeted therapeutic interventions and their chemical robustness for long-term bioimaging and diagnostics. The growing applications of squaraine-based functional materials in molecular sensing, bioimaging and theranostics pave the way for innovative advancements in diagnostics, personalized medicine and biomedical research."

Journal • Review

Tubular-Infiltrative Variant of Acinar Cell Carcinoma: Clinicopathologic Analysis of 11 Cases of A Distinct Entity Invariably Misdiagnosed as Ductal Adenocarcinoma (USCAP 2026) - "In our retrospective analysis, IHC showed trypsin positivity in 10/10, chymotrypsin 5/10 and CEL 5/7... We describe a [tubular-infiltrative] variant of ACC that exhibits many salient characteristics of PDACs and is thus uniformly misdiagnosed as PDAC. A constellation of morphologic findings including relative uniformity of the glands and cells, vague lobular architecture, prominence of nucleoli, and most importantly, intraluminal enzymatic concretions are diagnostic of ACC. IHC by acinar markers and absence of KRAS mutation, and BRAF/RAF alterations would also help point towards ACC."

Clinical • Oncology • Pancreatic Ductal Adenocarcinoma • BRAF • KRAS • RAF1

A Non-Peptidic Fluorescent Probe for In Vitro Detection of Chymotrypsin. (PubMed, ACS Appl Bio Mater) - "The probe showed effective performance in HepG2 cell models, confirming its suitability for biological applications. This study underscores the value of non-peptide probe designs for fast and precise enzyme detection in complex biological environments."

Journal • Preclinical

Enhancing antitumour response to proteasome inhibitors with inhibitors of insulin-degrading enzyme, a new molecular vulnerability in multiple myeloma. (PubMed, Br J Pharmacol) - "IDE emerges as a novel biomarker and therapeutic vulnerability in MM. This study demonstrates the relevance of IDE inhibitors as boosters of PIs to better treat patients with resistant MM."

Journal • Hematological Malignancies • Multiple Myeloma • Oncology • MYC

Caste-Specific Functional Variation in the Venom of the Army Ant Eciton hamatum. (PubMed, Mol Ecol) - "Chymotrypsin enzymes, more abundant in the venoms of soldier castes, may also serve a pre-digestive role. This pattern suggests an evolutionary trade-off favouring a limited set of highly effective components tailored to the dual function of defence and predation in soldiers."

Journal • Pain

Efficacy of chymotrypsin irrigation for endoscopically non-accessible walled-off necrosis in necrotizing pancreatitis. (PubMed, Endosc Int Open) - "No severe complications occurred and all patients completed the treatment course. Chymotrypsin irrigation effectively liquefies fibrotic necrotic tissue and promotes WON clearance via PCD, representing a promising adjunctive therapy for managing endoscopically non accessible WON."

Journal • Fibrosis • Gastrointestinal Disorder • Infectious Disease • Pancreatitis

Yeast enzyme hydrolysis slurry supplementation improves growth, intestinal health, and metabolic responses in juvenile largemouth bass (Micropterus salmoides) fed soybean meal-based diets with partial fishmeal replacement. (PubMed, J Anim Sci Biotechnol) - "Yeast enzyme hydrolysis slurry in soybean meal-based diets with partial fishmeal replacement enhanced the antioxidant capacity, reduced intestinal permeability, altered the abundances of intestinal microbiota and associated core metabolites. These positive changes collectively contributed to improved growth performance in largemouth bass."

Journal • CAT • LEP

The enzymes DisoNNex and TrypLE are interchangeable as harvesting agents for adipose tissue-derived mesenchymal stromal cells. (PubMed, Cytotherapy) - "DisoNNex is a relevant alternative to TrypLE for ASC harvest, resulting in equivalent yields and cell quality under the tested conditions. These findings support the potential of DisoNNex in GMP-compliant manufacturing of ASC-based cell therapies and provide starting conditions for its implementation in ASC processes. Nonetheless, full comparability studies within an established production process are pending."

IO biomarker • Journal

Capillary Electrophoresis Mass Spectrometry Interfacing via Multifunctional Vibrating Sharp Edge Ionization Spray for the Simultaneous Delivery of an Auxiliary Flow, Analyte Mixing, and Fluid Nebulization. (PubMed, Anal Chem) - "The direct CE-VSSI-MS interface is compatible with background electrolytes maintained at acidic or neutral pH and even composed of 200 mM ammonium acetate. Separations are demonstrated with cationic beta-blockers, amino acids, peptide standards, a peptide mixture from a chymotrypsin digestion of transferrin, and a 17-residue peptide monomer and homodimer derived from Huntington's protein that is implicated in protein aggregation."

Journal • CNS Disorders • Huntington's Disease • Movement Disorders

Assessing Virulence Factor Genes in Pig-Derived Escherichia coli from the Region of Vojvodina Treated with Postbiotic Substance and Herbal Essential Oils. (PubMed, Pathogens) - "Each E. coli strain produced the damaging enzyme, such as β-glucuronidase and/or α-chymotrypsin...The results contribute to the practical effectiveness of postbiotic substances, HEOs, and their combination as a novel approach to combating the virulence factors of E. coli. This insight also contributes to the strategy behind the One Health Concept."

Journal

Identification and localization of the set of serine peptidases and their homologs in the larval midgut of Tenebrio molitor L. (PubMed, Insect Mol Biol) - "Of the 17 SPs, 3 were trypsins, 10 chymotrypsin-like, and 4 elastase-like...The majority of analysed proteins had a high level of mRNA expression specific to the feeding stages of development. The results obtained suggest that T. molitor larvae possess a wide range of digestive SPs and SPHs, which may provide them with high resistance to numerous dietary or insecticidal SP inhibitors, as well as indicate wide possibilities for their use for biotechnological production of T. molitor food/feed hydrolysates of this edible insect with increased digestibility, reduced allergenicity and possibly new biological activities."

Journal • ELANE

Pancreatitis-associated chymotrypsin C (CTRC) variant p.R240Q selectively impairs trypsinogen degradation through disruption of long-range electrostatic interactions. (PubMed, Sci Rep) - No abstract available

Journal • Pancreatitis • CTRC

Rapid Histone Post-Translational Modification Analysis Using Alternative Proteases and Tandem Mass Tags. (PubMed, bioRxiv) - "Through systematic evaluation of the Arg-C Ultra protease and a prototype recombinant (r)-Chymotrypsin protease under varied conditions, such as chemical derivatization using propionic anhydride and tandem mass tags (TMT), we demonstrated that Arg-C Ultra with TMT labeling achieves a detection of total PTM comparable to conventional Trypsin-based approaches...Application of RIPUP to frozen-thawed rat hippocampal sections within a 3-hour workflow identifies >200 PTMs including biologically critical PTM sites H3 K27/K36/K37 methylation, H4 N-terminal acetylation patterns, and H2A ubiquitination at K118/K119. This rapid, high-efficiency platform enables timely discovery of epigenetic mechanisms and accelerates the path from PTM identification to therapeutic target validation."

Journal • Targeted Protein Degradation

Residue-Specific Signatures of Structural Water Identified by Dissolution Dynamic Nuclear Polarization with UV-Generated Radicals. (PubMed, Angew Chem Int Ed Engl) - "In the model protein chymotrypsin inhibitor 2, HyperW-enhanced two-dimensional NMR correlation spectra across pD 5.5-8.4 reveal strong exchange-driven enhancements at solvent-exposed residues...The CLEANEX-negative/HyperW-positive signature, together with the spatial proximity of these residues to crystallographically conserved water molecules, supports NOE-mediated transfer from long-residence internal water, not distinguishable by standard NMR methods. The combined observables establish HyperW NMR as a residue-specific reporter of structural hydration and hydration-coupled dynamics under native conditions, providing a route to link conserved water observed in crystals to their roles in solution."

Journal

Functional characterization of secreted Schistosoma mansoni serpin SmSPI reveals Factor XIIa inhibition as evidenced by biochemical, and human plasma assays and supported by AI-assisted structural modelling. (PubMed, Int J Parasitol) - "The resulting in silico predictions were validated by enzymatic activity assays, which demonstrated that recombinant SmSPI effectively inhibited several host proteases, including cathepsin G, urokinase, and pancreatic elastase, with weaker inhibition observed against chymotrypsin...These results establish SmSPI as a multifunctional host-interacting serpin with immunomodulatory and targeted anticoagulant properties. Notably, its ability to inhibit FXIIa identifies SmSPI as the first helminth-derived inhibitor of the contact activation system, making the protein a promising candidate for future anticoagulant therapies designed to limit thrombosis while preserving normal hemostasis."

Journal • Cardiovascular • Hematological Disorders • Inflammation • Thrombosis • CTSG • ELANE

Bacillus velezensis CML532 supplementation improved growth performance, protein digestion, and gut health in broilers fed with corn-miscellaneous meal-based low-protein diets. (PubMed, Anim Nutr) - "In the jejunum, the CMLB group significantly increased the expression of intestinal epithelial barrier proteins, and elevated the activities of α-amylase and chymotrypsin in the digesta compared with both the CM and CML groups (P < 0.05)...Ileal microbial analysis revealed a decreased relative abundance of uncultured_bacterium_g_Lactobacillus in the CML group, whereas B. velezensis supplementation increased the enrichment of Coriobacteriaceae _bacterium_CHKCI002 and B. velezensis, accompanied by enhanced His metabolism and biosynthesis pathways. These results indicated that B. velezensis CML532 supplementation improved protein digestion and utilization by enhancing digestive enzyme activity and modulating gut microbial composition, thereby promoting growth performance and intestinal health in broilers fed with low-protein corn-miscellaneous meal-based diets to levels comparable with those fed normal protein diets."

Journal • GPRC6A • IL10

Alterations in the CTRB2 gene and response to chemotherapy in pancreatic cancer. (PubMed, PLoS One) - "No clinical impact on chemotherapy response or OS was observed by CTRB2 deletion status. Further studies are needed to identify reliable biomarkers of therapy response in PDAC."

Journal • Retrospective data • Oncology • Pancreatic Cancer • Pancreatic Ductal Adenocarcinoma • Solid Tumor

Peptide Mapping Using Multienzyme Digestion Strategies Integrated with LC-HRMS Workflow: A Case Study. (PubMed, J Pept Sci) - "Full sequence coverage of both peptides was achieved by combining peptide maps produced with trypsin, Glu-C, and chymotrypsin. Application of the workflow to a GLP-1 analog provided > 95% sequence coverage and accurate intact-mass confirmation. Overall, the described method offers a reliable and stepwise approach for peptide-mapping-based structural verification, enabling confident assessment of the primary structure of exenatide and H-GLP-1."

Journal

Rice Bran-Derived Peptides with Antioxidant Activity: Effects of Enzymatic Hydrolysis Using Bacillus licheniformis and α-Chymotrypsin. (PubMed, Foods) - "Enzymatic hydrolysis enhanced total phenolic content compared with native rice bran protein. These findings highlight the potential of rice bran-derived peptides as antioxidant candidates and indicate that further validation in food systems is required."

Journal

Combined loss of APC and Id3 in pancreatic acinar cells causes pleiotropic phenotypes in a genetically engineered murine model (ACMG 2026) - " Methods To address possible mechanisms behind PACC tumor formation, we generated a mouse model featuring an acinar cell specific knockout (KO), directed by the chymotrypsin-like elastase family, member 1 ( Cela1 ) promoter-driven Cre recombinase... Conclusions These results highlight the complex role of Id3 and APC dysregulation in PACC tumorigenesis, as KO of both genes in acinar cells is not sufficient for PACC development in mice. Future experiments aim to elucidate how dual loss of Id3 and APC leads to various phenotypes through use of omics and imaging techniques such as RNA-seq, Assay for Transposase-Accessible Chromatin with sequencing (ATAC-seq), and biomarker analyses by immunofluorescence, and immunohistochemistry."

Preclinical • Leukopenia • Pancreatic Cancer • Pancreatic Ductal Adenocarcinoma • Solid Tumor • Thrombocytopenia • ELANE • ID3

Thermal stability and hydration dynamics of Chymotrypsin Inhibitor 2 in aqueous amino acid solutions from replica exchange molecular dynamics. (PubMed, Biochem Biophys Res Commun) - "Radial distribution and cluster analyses indicated that arginine formed compact clusters near the protein surface, whereas glutamic acid remained dispersed, allowing higher water mobility. Water diffusion and dipole-dipole correlation studies revealed faster hydration dynamics in glutamic acid and slower, more confined motion in arginine, with intermediate behavior in the mixed solution."

Journal

LC-MS peptide mapping of monoclonal antibodies using the mirror proteases trypsin and Tryp-N. (PubMed, J Pharm Biomed Anal) - "As a result, supplementary endoproteases such as Asp-N, chymotrypsin, Glu-C or Lys-C are often employed to enhance coverage...The sequence coverages achieved for bevacizumab, cetuximab, NISTmAb, and trastuzumab with Tryp-N were comparable to that of trypsin, and the combination of both enzymes slightly improved overall sequence coverage...The presence of a basic amino acid at opposite ends of the peptide often resulted in complementary sequence coverage of the MS2 of the same peptide sequences. These complementary ion series can be leveraged for precise localization of PTMs, as demonstrated in detail for deamidation, and oxidation sites as well as single amino acid variants (SAVs)."

Journal • Targeted Protein Degradation