VSEL (very small embryonic-like stem cells) / Lisata Therap - LARVOL DELTA

Efficient Ex Vivo Expansion of Human Umbilical Cord Blood-Derived Small CD133+lin-CD45- Stem Cells Expressing Pluripotency Markers in Presence of Histone Deacethylase Inhibitors - As a New Challenging Strategy to Obtain Multipotent Cells for Regenerative Medicine (ASH 2017) - "This data also support a hypothetical link between primordial germ cells and hematopoiesis. We propose that the currently established expansion system for VSELs isolated from postnatal tissues provides a new and exciting opportunity to employ cells isolated from adult tissues in regenerative medicine."

Biosimilar • Hematological Malignancies • Oncology

Human Very Small Embryonic-Like Stem Cells Are Present in Normal Peripheral Blood of Young, Middle-Aged, and Aged Subjects. (PubMed) - Stem Cells Int - "The difference in mRNA expression for PSC markers as SSEA-4, Oct-4, Nanog, and Sox2 between the three groups of age was not statistically significant. A similar frequency of huVSELs into the SS-PB of young, middle-aged, and aged subjects may indicate that the VSELs pool persists all along the life as a reserve for tissue repair in case of minor injury and that there is a continuous efflux of these cells from the BM into the PB."

Journal • Biosimilar

Novel View on Umbilical Cord Blood and Maternal Peripheral Blood-an Evidence for an Increase in the Number of Circulating Stem Cells on Both Sides of the Fetal-Maternal Circulation Barrier. (PubMed, Stem Cell Rev) - "We observed that the number of HSCs, MSCs, EPCs, and VSELs increases in maternal blood at 24 h after physiological delivery (n = 17). Based on this observation, we propose that delivery stress is associated with an increase in the number of circulating stem cells, not only on the fetal side but also on the maternal side of the fetal-maternal circulatory barrier."

Journal • Biosimilar

Being Pluripotent, Bone Marrow VSELs Rather than HSCs have the Potential to Regenerate other Adult Organs. (PubMed, Stem Cells) - "HSCs also do not exhibit the ability to transdifferentiate or be reprogrammed to any other cell fate. The true regenerative ability lies with this primitive and novel population of pluripotent stem cells in the BM termed very small embryonic-like stem cells (VSELs) which were most likely discarded by Deanne's group while processing cells for transplantation."

Clinical • Journal • Review • Biosimilar

Mobilization of Peripheral Blood Stem Cells and Changes in the Concentration of Plasma Factors Influencing their Movement in Patients with Panic Disorder. (PubMed) - "The level of VSELs (Lin-/CD45-/CD133 +) was significantly lower in the patient group before treatment as compared to the patient group after treatment.The levels of factors responsible for stem cell movement were significantly lower in the patient group compared to the control group before and after treatment. It was concluded that the study of stem cells and factors associated with their movement can be useful in the diagnostics of panic disorder, as well as differentiating between psychotic and anxiety disorders."

Journal • Biosimilar • CNS Disorders

VSELs may obviate cryobanking of gonadal tissue in cancer patients for fertility preservation. (PubMed) - J Ovarian Res - "Endogenous VSELs (which survive oncotherapy) can possibly regenerate non-functional gonads in cancer survivors when exposed to a healthy niche in vitro or in vivo (by way of transplanting mesenchymal cells which secrete trophic factors required for endogenous VSELs to differentiate into gametes). Presence of VSELs can also explain spontaneous pregnancies after BMT and cortical tissue transplantation (at heterotopic or orthotopic sites). This understanding once verified and accepted by the scientific community could obviate the need to remove whole ovary or testicular biopsy for cryopreservation prior to oncotherapy."

Journal • Biosimilar • Inflammation • Oncology

Very small embryonic-like stem cells (VSELs) detected in azoospermic testicular biopsies of adult survivors of childhood cancer. (PubMed) - Reprod Biol Endocrinol - "The present study demonstrates the presence of pluripotent VSELs in the testicular biopsy of azoospermic adult survivors of childhood cancer. It is likely that these persisting VSELs can restore spermatogenesis as demonstrated in mice studies. Therefore, pilot studies need to be undertaken using autologous mesenchymal cells with a hope to restore testicular function and fertility in cancer survivors. The results of this study assume a great significance in the current era, where cryopreservation of testicular tissue in young pre-pubertal boys for restoring spermatogenesis in adulthood is still in experimental stages."

Journal • Biosimilar • Oncology

Ovarian stem cells are always accompanied by very small embryonic-like stem cells in adult mammalian ovary. (PubMed) - J Ovarian Res - "VSELs have remained poorly studied till now because of their very small size and rare occurrence. Spinning cells obtained after enzymatic digestion of ovarian tissue at a speed of 1000G (rather than 1200 rpm) throughout processing allows reliable detection of the VSELs by flow cytometry. VSELs exist in aged, chemoablated and non-functional ovary and providing a healthy niche to support their function offers an interesting strategy to manage infertility."

Journal • Biosimilar