CSA-131 / N8 Medical - LARVOL DELTA

Bactericidal activity of ceragenin CSA-13, CSA-44 and CSA-131 against bacteria causing bloodstream infections. (PubMed, Front Microbiol) - "Minimum inhibitory concentrations/minimum bactericidal concentrations (MIC/MBC) against clinical bacterial strains were measured and compared with activity of clinically used antibiotics colistin and vancomycin. Results presented herein demonstrate the antibacterial activity of ceragenins against clinical strains of bacteria isolated from blood, their influence on the immune system and the integrity of the endothelial cell monolayer. Further studies are necessary to understand the cell signaling pathway governing these effects."

Journal • Hematological Disorders • Infectious Disease

Bottom-up sono-enzymatic approach to build antimicrobial and antifouling nano-enabled coatings on urinary catheters. (PubMed, Nanoscale Adv) - "Ceragenin CSA-131, an antimicrobial peptide, and lauryl gallate were nanoformulated into colloidal nanoparticles using high-intensity ultrasound...No adverse effects on fibroblast viability were observed over the same period of time in contact with the coated silicones, demonstrating their excellent biocompatibility (>90%). Altogether, this sono-enzymatically engineered, bio-based coating offers a promising strategy to reduce bacterial colonisation and biofilm formation on urinary indwelling devices and consequently the risk of infection in catheterised patients."

Journal • Infectious Disease

Ceragenin Nanogel Coating Prevents Biofilms Formation on Urinary Catheters. (PubMed, ACS Appl Mater Interfaces) - "The nanogel comprising biocompatible gum arabic and poly(diallyldimethylammonium chloride) was used to encapsulate a synthetic broad-spectrum antimicrobial peptide mimetic ceragenin (CSA-131)...Cytotoxicity assessment confirmed the absence of toxic effects, underscoring the biocompatibility of the coating formulation. These findings highlighted the potential of nanogels, combined with ultrasound technology, as an innovative approach for durable antimicrobial and antibiofilm functionalization of urinary catheters, particularly susceptible to colonisation by microorganisms upon catheterization."

Journal • Infectious Disease • Nephrology

Investigating the Effectiveness of Ceragenins against Acinetobacter baumannii to Develop New Antimicrobial and Anti-Adhesive Strategies. (PubMed, Int J Mol Sci) - "Assessment of the bactericidal effect of ceragenins CSA-13, CSA-44, and CSA-131 on clinical isolates of A. baumannii (n = 65) and their effectiveness against bacterial cells embedded in the biofilm matrix after biofilm growth on abiotic surfaces showed a strong bactericidal effect of the tested molecules regardless of bacterial growth pattern...In the cell culture of A549 lung epithelial cells, ceragenin CSA-13 had the ability to inhibit bacterial adhesion to host cells, suggesting that it interferes with the mechanism of bacterial cell invasion. These findings highlight the potential of ceragenins as therapeutic agents in the development of antimicrobial strategies against bacterial infections caused by A. baumannii."

Journal • Infectious Disease

Activities of CSA-44 and CSA-131 against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in collagen wound biofilm and Caenorhabditis elegans model systems (ECCMID 2024) - No abstract available

Searching the potential application of CSA-131 as a coating material for implant surfaces (EUROBIOSIMILARS 2024) - No abstract available

Sono-enzymatic functionalization on urinary catheters with antifouling and antimicrobial nano-enabled hydrogel coating (ACS-Sp 2024) - "Ceragenin CSA-131, a cholic acid-based mimic of antimicrobial peptides, was loaded in lauryl gallate nanoparticles (CSA@LG NPs) which were then deposited on urinary catheter using high-intensity ultrasound coupled with laccase-mediated grafting to stable antibacterial coating...This coating was stable for up to 7 days under conditions simulating the real scenario of catheter usage and inhibited by 90 % P. aeruginosa and S. aureus biofilms. Importantly, these nano-enabled coatings did not compromise the viability of human cells, even after a 7 days of contact."

Immunology • Infectious Disease • Inflammation • Nephrology

Efficacy of Ceragenins in Controlling the Growth of Oral Microorganisms: Implications for Oral Hygiene Management. (PubMed, Pharmaceuticals (Basel)) - "We demonstrate that ceragenins CSA-13, CSA-44, and CSA-131 as well as undescribed to date CSA-255 display potent antimicrobial activities against isolates of fungi, aerobic, and anaerobic bacteria from Candida, Streptococcus, Enterococcus, and Bacteroides species, which are well-recognized representatives of microbes found in the oral cavity...While the ceragenins showed similar or slightly diminished efficacy compared to commercially available mouthwashes, they demonstrated a highly favorable toxicity profile toward host cells, that may translate into better maintenance of host mucosal membrane stability. This suggests that incorporating ceragenins into oral hygiene products could be a valuable strategy for reducing the risk of both oral cavity-localized and secondary systemic infections and for improving the overall health outcomes of individuals receiving medical treatment."

Journal • Infectious Disease

Antimicrobial Activity of Ceragenins against Vancomycin-Susceptible and -Resistant Enterococcus spp. (PubMed, Pharmaceuticals (Basel)) - "In this study, the antibacterial activities of various ceragenins (CSA-13, CSA-44, CSA-90, CSA-131, CSA-138, CSA-142, and CSA-192), linezolid, and daptomycin were assessed against 50 non-repeated Enterococcus spp. Compared to no treatment, increased survival was observed with C. elegans when treated with ceragenins. In conclusion, CSA-44 and CSA-192 appear to be good candidates (alone or in combination) for the treatment of enterococcal infections, including those from VRE."

Journal • Infectious Disease

Ceragenins exhibit bactericidal properties that are independent of the ionic strength in the environment mimicking cystic fibrosis sputum. (PubMed, Front Microbiol) - "CF-associated bacterial (Pseudomonas aeruginosa, Ochrobactrum spp., and Staphylococcus aureus), and fungal pathogens (Candida albicans, and Candida tropicalis) were used as target organisms for ceragenins (CSA-13 and CSA-131) and AMPs (LL-37 and omiganan). Our results demonstrated that ceragenins exhibit potent antimicrobial activity in CF sputum regardless of the NaCl concentration when compared to LL-37 and omiganan. Given the broad-spectrum antimicrobial activity of ceragenins in the microenvironments mimicking the airways of CF patients, ceragenins might be promising agents in managing CF disease."

Journal • Cystic Fibrosis • Fibrosis • Genetic Disorders • Immunology • Infectious Disease • Pulmonary Disease • Respiratory Diseases

Phenotypic Investigation of Virulence Factors, Susceptibility to Ceragenins, and the Impact of Biofilm Formation on Drug Efficacy in Candida auris Isolates from Türkiye. (PubMed, J Fungi (Basel)) - "Upon determining the minimum inhibitory concentrations (MIC) of ceragenins, it was discovered that CSA-138 exhibited the highest effectiveness with a MIC range of 1-0.5 µg/mL, followed by CSA-131 with a MIC of 1 µg/mL...The investigation revealed that the strains isolated from Türkiye displayed weak exoenzyme activities. Notably, the ceragenins exhibited effectiveness against these strains, suggesting their potential as a viable treatment option."

Journal • Critical care • Hematological Disorders

Incorporating Ceragenins into Coatings Protects Peripherally Inserted Central Catheter Lines against Pathogen Colonization for Multiple Weeks. (PubMed, Int J Mol Sci) - "We have previously developed synthetic mimics of ubiquitous antimicrobial peptides and have worked to apply a lead compound, CSA-131, to the crisis...Microbial challenges by methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Candida albicans were performed and SEM was used to evaluate coating structure and colonization. The results indicate that selected coatings show activity against selected planktonic pathogens that extend between 16 and 33 days, with similar periods of biofilm prevention."

Journal • Infectious Disease • Pneumonia

Efficacy of Ceragenins Alone and in Combinations with Antibiotics Against Multidrug-Resistant Gram Negative Pathogens from Bloodstream Infections. (PubMed, Curr Microbiol) - "This study investigated the antibacterial activities of eight different ceragenins against MDR pathogens and the synergistic effects of some ceragenins in combinations with antibiotics (meropenem-MEM, ceftazidime + avibactam-CZA, tigecycline-TIG). Antagonism was not detected in any of the combinations. CSA-44 and CSA-131 alone/or in combinations with MEM or CZA can be considered as new alternative treatments in serious infections caused by MDR pathogens."

Gram negative • Journal • Infectious Disease • Pneumonia

FDA grants QIDP Designation For Kinnear's CSA-131 drug for Life-threatening Pseudomonas Bacterial Infections in CF (EIN Presswire) - "Kinnear Pharmaceuticals, LLC, a subsidiary of N8 Medical, Inc. today announced the U.S. Food and Drug Administration (FDA) has granted Qualified Infectious Disease Product (QIDP) Designation for CSA-131 for the prevention and treatment of life threatening Pseudomonas infections in patients with Cystic Fibrosis."

FDA event • Cystic Fibrosis • Infectious Disease