P306 / Fox Chase Cancer Center - LARVOL DELTA

PC306. Is Albumin Safe For Patients Undergoing Craniotomy? (ASA 2024) - "Learning Objective 2 Describe risks to patients that might be associated with intravenous albumin in those with traumatic brain injury. Learning Objective 3 Formulate a plan for intravascular volume replacement in patients undergoing craniotomy"

Clinical • CNS Disorders • Vascular Neurology

PN306. Tools vs. Toys: Using POCUS to Improve Patient Care In Pediatric Anesthesia (ASA 2024) - "Learning Objective 2 The attendee should be able to explain how PoCUS can be used to reduce cancellations in the setting of some NPO violations.. Learning Objective 3 The attendee should be able to describe how PoCUS can be used to evaluate the lungs in modern anesthesia practice."

Clinical • Anesthesia • Pediatrics

PS306. The Fundamentals of Communication in Surgery (ACS-CLINCON 2024) - "• Articulate novel strategies to engage patients in decision making. • Consider actions you can do in your practice now to improve communication about surgery."

Surgery • Palliative care

Subcutaneous administration of isatuximab by an on-body delivery system (OBDS) in multiple myeloma patients: results of a nurse survey (IMW 2024) - P1 | "Introduction: Intravenous (IV) isatuximab (Isa) in combination with pomalidomide-dexamethasone (Pd) is approved for the treatment of relapsed/refractory multiple myeloma (RRMM) patients...Extent of drug delivered not clear in case of interruption (83%) and need to press/hold a button to pause injection (67%) were agreed upon as potential disadvantages for HCPs; risk of allergy to the adhesive tape (50%) was reported as a potential disadvantage for patients, although no interruptions or allergy were observed in 581 administrations via OBDS in the Phase 1b study (IMS 2023; P-306). Findings from this nurse survey show a high level of confidence and preference for SC Isa treatment delivery using a hands-free OBDS vs IV administration, due to its ease of use, tolerability, and time savings, suggesting its applicability in routine clinical practice."

Clinical • Hematological Malignancies • Immunology • Multiple Myeloma • Oncology • Pain

PS306. The Top New Pediatric Trauma Guidelines and Algorithms: A Multisociety Collaborative Review and Discussion (ACS-CLINCON 2023) - "5. Discuss the guidelines in the diagnosis and initial management of children with suspected non-accidental trauma."

Clinical • Review • Cardiovascular • Pediatrics • Venous Thromboembolism

PN306. A Case Based Discussion of Adult Congenital Heart Disease – Tips & Tricks from the Experts (ASA 2023) - "Anesthesiologists are encountering congenital cardiac patients in various procedural locations (e.g., OB, ambulatory centers, trauma orthopedic fractures, cardiac surgery). We will lead case-based discussions on different cardiac lesions, including different repairs/palliation and complications, and important aspects to consider for anesthetic management."

Clinical • Anesthesia • Cardiovascular • Heart Failure • Musculoskeletal Diseases • Orthopedics

AC.APS.P306. Unique Populations in ACS: So Much to Be Learnt (AHA 2022) - No abstract available

Clinical • Cardiovascular

Imaging single CaMKII holoenzymes at work by high-speed atomic force microscopy. (PubMed, Sci Adv) - "Among the species studies, only rat CaMKIIα with pT286/pT305/pT306 exhibited kinase domain oligomerization. Furthermore, we revealed that the sensitivity of CaMKII to PP2A in the three species differs, with rat, C. elegans, and hydra being less dephosphorylated in that order. The evolutionarily acquired features of mammalian CaMKIIα-specific structural arrangement and phosphatase tolerance may differentiate neuronal function between mammals and other species."

Journal

T-bet controls the maintenance and differentiation potential of flu-induced effector memory B cells (P306) (IMMUNOLOGY 2023) - "Using constitutive and inducible models to delete Tbx21 specifically in B cells we showed that the rapid differentiation potential of Bmem is controlled by T-bet and that T-bet is required for the development and persistence of NP+ eBmem subsets in LN and lung. Thus T-bet not only marks Bmem with effector potential but also regulates the persistence and function of flu-induced NP+ Bmem that are transcriptionally poised to provide rapid humoral protection from infection."

Clinical • Infectious Disease • Influenza • Respiratory Diseases • CD27 • TBX21

Bruton’s tyrosine kinase impacts IgA repertoire and microbiome composition in non-obese diabetic mice (P306) (IMMUNOLOGY 2023) - "BTK-deficient mice have increased commensal diversity and enrichment of specific microbes. Based on these findings, we conclude that disruption of BTK-mediated BCR signaling alters IgA production and microbiome composition, with downstream effects on T1D pathogenesis."

Preclinical • CNS Disorders • Diabetes • Metabolic Disorders • Obesity • Type 1 Diabetes Mellitus • BTK

Testing of a novel liposomal anti-Pseudomonas vaccine based on outer-membrane protein F (OprF) of Pseudomonas aeruginosa (P306) (IMMUNOLOGY 2023) - "The mice immunized with the modified vaccine elicit higher antibody titers and increased IgG2a antibodies. Overall, our liposomal vaccines were found to be highly effective at generating a balanced and robust TH1/TH2 response."

Cystic Fibrosis • Fibrosis • Genetic Disorders • Immunology • Infectious Disease • Pulmonary Disease • Respiratory Diseases • TLR2

PS306. Peroral Endoscopic Myotomy (POEM) for Achalasia in Adolescents (ACS-CLINCON 2022) - "The surgical management of achalasia in children is most commonly addressed with a laparoscopic Heller myotomy; however, the advent of POEM and the advancement of this technology has presented other options in younger patients. This session will discuss “best practice” approaches to achalasia in children and adolescents including technical pointers related to laparoscopic Heller myotomy as well as the emerging role of POEM for adolescents."

Gastrointestinal Disorder • Rare Diseases

PN306. Towards Controlling the Chaos: Organization and Scheduling of Cardiac Services Outside of the Operating Room (ASA 2021) - "Construct a coverage model for a growing electrophysiology service. Diagram the financial implications of out-of OR anesthesia coverage."

Anesthesia

Mutability of mononucleotide repeats, not oxidative stress, explains the discrepancy between laboratory-accumulated mutations and the natural allele-frequency spectrum in C. elegans. (PubMed, Genome Res) - "Using genome sequence data from C. elegans MA lines carrying a mutation (mev-1) that increases the cellular titer of reactive oxygen species (ROS), leading to increased oxidative stress, we find the base-substitution spectrum is similar between mev-1, its wild-type progenitor (N2), and another set of MA lines derived from a different wild strain (PB306)...Further, the mutational properties of mononucleotide repeats in all strains are different from those of nonmononucleotide sequence, both for indels and base-substitutions, and whereas the nonmononucleotide spectra are fairly similar between MA lines and wild isolates, the mononucleotide spectra are very different, with a greater frequency of A:T → T:A transversions and an increased proportion of ±1-bp indels. The discrepancy in mutational spectra between laboratory MA experiments and natural variation is likely owing to a consistent (but unknown) effect of the laboratory environment that manifests itself via..."

Journal

Structural Variation and Chemical Performance-A Study of the Effects of Chemical Structure upon Epoxy Network Chemical Performance. (PubMed, ACS Appl Polym Mater) - "To achieve this, four industrially relevant epoxy resins (two based on bisphenol A-Epikote 828 (E828) and Dow Epoxy Resin 332 (DER 332)-and two based on bisphenol F-Dow Epoxy Resin 354 (DER 354) and Araldite PY306 (PY306)) and the isomerically pure para-para-diglycidyl ether of bisphenol F (ppDGEBF) were used to explore variation caused by epoxy monomer variation...Bisphenol F-based resins were found to outperform bisphenol A-based analogues, and chain extension within the epoxy component was found to be detrimental to performance. For amines, 1,3-substitution (vs 1,4) and aromaticity were both found to be beneficial to chemical performance."

Journal

[VIRTUAL] A Unique Loop Structure in the A2-domain of Pseudonaja textilis Factor Va Prevents Activated Protein C-catalyzed A2-domain Dissociation (ISTH 2021) - "Methods : The human Arg306-region PKKTRNL was exchanged for the homologous ptFV sequence GNPDTLT, generating ptFV-h306 and hFV-pt306; recombinant removal of ptFV’s disulfide bond resulted in ptFV-h306-SS...Remarkably, upon proteolysis the structural integrity of ptFVa is maintained, indicating a completely novel role for specific A1 and A2-domain structural regions that have been uniquely adapted in ptFVa in order to prevent A2-domain dissociation. As such, these results challenge our current understanding by which strict regulatory mechanisms control FVa activity."

Targeted Protein Degradation

[VIRTUAL] PN306. Implantable Devices and Anesthesia: Neuraxial, Regional and Intraoperative/Perioperative Considerations (ASA 2020) - "Session Learning Objective 2 Develop anesthetic plans, when neuraxial anesthesia is potentially necessary, in patients with spinal cord stimulators and intrathecal drug delivery devices. Session Learning Objective 3 Evaluate factors that may impact efficacy, safety of regional anesthesia and patients with existing peripheral nerve stimulators."

Anesthesia • Pain

The nematode Caenorhabditis elegans and the terrestrial isopod Porcellio scaber likely interact opportunistically. (PubMed, PLoS One) - "We show that two different strains-the wild isolate PB306 and the laboratory-adapted strain N2 -are not attracted to P. scaber during either the dauer or adult life stages. Our results indicate that C. elegans was not attracted to chemical compounds or volatile odorants from P. scaber, providing valuable empirical evidence to suggest that any associations between these two species are likely opportunistic rather than specific phoresy."

Journal

PG306 Evidence-Based Guidance on Telehealth Interventions to Improve Health Outcomes in Obstetrics and Gynecology (ACOG 2020) - No abstract available

HEOR • Gynecology • Obstetrics

PN306 Hot, Important and Controversial Topics in Ambulatory and Office-Based Anesthesia (ASA 2018) - "Review the higher risk surgical procedures in discussion of whether there are some procedures not appropriate for an office based setting. Describe the risks associated with providing ambulatory anesthesia to the geriatric patient and verbalize whether there should be an age cut off."

Anesthesia • Biosimilar • Metabolic Disorders • Obesity

Post-translational modifications of Drosophila melanogaster HOX protein, Sex combs reduced. (PubMed, PLoS One) - "The PTMs identified include phosphorylation at S185, S201, T315, S316, T317 and T324, acetylation at K218, S223, S227, K309, K434 and K439, formylation at K218, K309, K325, K341, K369, K434 and K439, methylation at S19, S166, K168 and T364, carboxylation at D108, K298, W307, K309, E323, K325 and K369, and hydroxylation at P22, Y87, P107, D108, D111, P269, P306, R310, N321, K325, Y334, R366, P392 and Y398...SLiMs are proposed to be preferential sites of phosphorylation. Although 6 of 7 phosphosites map to regions of predicted SLiMs, we find no support for the hypothesis that the individual S, T and Y residues of predicted SLiMs are phosphorylated more frequently than S, T and Y residues outside of predicted SLiMs."

Journal

The Chk1 inhibitor SAR-020106 sensitizes human glioblastoma cells to irradiation, to temozolomide, and to decitabine treatment. (PubMed, J Exp Clin Cancer Res) - "The Chk1 inhibitor SAR-020106 is a potent sensitizer for DNA damage-induced cell death in glioblastoma therapy strongly reducing clonogenicity of tumour cells. Selectively enhanced p53-mut cell death may provide stronger responses in tumours defective of non-homologous end joining (NHEJ). Our results suggest that a multimodal therapy involving DNA damage inducers and DNA repair inhibitors might be an effective anti-tumour strategy with a low risk of neurotoxicity."

Journal

"Interested by PTH action on cTAL transport properties and Claudin regulation, do not miss Poster SA-PO306 Saturday morning at #KidneyWeek2019 presented by Caroline Bertoye !!!" (@et_rein)

Pseudonaja Textilis Factor Va Retains Structural Integrity and Cofactor Function Following Proteolysis by Activated Protein C (ISTH 2019) - "... The non-conserved ptFV sequence GNPDTLT replacing the Arg306-like region was exchanged for the homologous human Arg306 sequence PKKTRNL, generating ptFV-h306 and hFV-pt306... Collectively, these findings indicate that, conversely to human FVa, APC-proteolysis of ptFVa at Arg306 or deletion of the unique disulfide bond does not abrogate cofactor function. Concomitantly, the structural integrity of ptFVa is stabilized, preventing A2-domain dissociation, thereby providing productive interactions. Additional support comes from plasmin-mediated proteolysis, suggesting an enhanced stability of essential functional regions in ptFVa ."