GBR 900 / Glenmark - LARVOL DELTA

ZmbZIP39 coordinates source-flow-sink relations to boost grain filling in maize. (PubMed, Plant Cell) - "ZmbZIP39 also functions within a higher-order regulatory complex, synergizing with key factors such as ZmABI19, ZmbZIP29, OHP1, OHP2, and ZmNAC130. ZmbZIP39 autoactivation activity creates a positive feedback loop that strengthens its downstream network. Our findings establish ZmbZIP39 as a central coordinator of source-flow-sink relations."

Journal

The biosynthesis of storage reserves and auxin is coordinated by a hierarchical regulatory network in maize endosperm. (PubMed, New Phytol) - "In this study, we identified 1744 high-confidence target genes co-regulated by the transcription factors (TFs) ZmNAC128 and ZmNAC130 (ZmNAC128/130) through chromatin immunoprecipitation sequencing coupled with RNA-seq analysis in the zmnac128/130 loss-of-function mutants...The hierarchical regulatory network centered by ZmNAC128/130 revealed a significant overlap between the direct target genes of ZmNAC128/130 and their downstream TFs, particularly in regulating the biosynthesis of storage reserves and IAA. Our results indicated that the biosynthesis of storage reserves and IAA is coordinated by a multi-TFs hierarchical regulatory network in maize endosperm."

Journal

Central Roles of ZmNAC128 and ZmNAC130 in Nutrient Uptake and Storage during Maize Grain Filling. (PubMed, Genes (Basel)) - "In addition to their direct involvement in sugar transport, ZmNAC128 and ZmNAC130 also activate the expression of the endosperm-preferential nitrogen and phosphate transporters ZmNPF1.1 and ZmPHO1;2. This coordinated regulation limits the intake of HMMs, enhances biofortification, and facilitates the uptake and storage of essential nutrients."

Journal • HK1

The transcription factors ZmNAC128 and ZmNAC130 coordinate with Opaque2 to promote endosperm filling in maize. (PubMed, Plant Cell) - "ZmNAC128 and ZmNAC130 regulate the expression of the transporter genes sugars will eventually be exported transporter 4c (ZmSWEET4C), sucrose and glucose carrier 1 (ZmSUGCAR1), and yellow stripe-like2 (ZmYSL2) and in turn facilitate nutrient uptake, while O2 plays a supporting role. In conclusion, ZmNAC128 and ZmNAC130 cooperate with O2 to facilitate endosperm filling, which involves nutrient uptake in the basal endosperm transfer layer (BETL) and the synthesis of zeins and starch in starchy endosperm (SE)."

Journal

The NAC transcription factor ZmNAC132 regulates leaf senescence and male fertility in maize. (PubMed, Plant Sci) - "Moreover, ZmNAC132 affected male fertility through the upregulation of ZmEXPB1, an expansin-encoding gene associated with sexual reproduction and other related genes. Together, the results show that ZmNAC132 participates in the regulation of leaf senescence and male fertility through the targeting of different downstream genes in maize."

Journal

NAC-type transcription factors regulate accumulation of starch and protein in maize seeds. (PubMed, Proc Natl Acad Sci U S A) - "Given that both the Bt2 and the 16-kDa γ-zein genes were activated by ZmNAC128 or ZmNAC130, we could identify a core binding site ACGCAA contained within their target promoter regions by combining Dual-Luciferase Reporter and Electrophoretic Mobility Shift assays. Consistent with these properties, transcriptomic profiling uncovered that lack of ZmNAC128 and ZmNAC130 had a pleiotropic effect on the utilization of carbohydrates and amino acids."

Journal