biglycan (TVN-102) / Tivorsan - LARVOL DELTA

Effect of anti-IL17 and/or Rho-kinase inhibitor treatments on vascular remodeling induced by chronic allergic pulmonary inflammation. (PubMed, Ther Adv Respir Dis) - "Sixty-four BALB/c mice, with pulmonary inflammation induced by ovalbumin were treated with anti-IL17A (7.5/µg per dose, intraperitoneal) and/or Rho-kinase inhibitor (Y-27632-10 mg/kg, intranasal), 1 h before each ovalbumin challenge (22, 24, 26, and 28/days)...We observed no difference in angiogenesis after treatment with Rho-kinase inhibitor and anti-IL17. Although the treatments did not show differences in angiogenesis, they showed differences in the markers involved in the angiogenesis process contributing to inflammation control and vascular remodeling.The reviews of this paper are available via the supplemental material section."

Journal • Asthma • Immunology • Inflammation • Pneumonia • Respiratory Diseases

Smooth Muscle Cell-Proteoglycan-Lipoprotein Interactions as Drivers of Atherosclerosis. (PubMed, Handb Exp Pharmacol) - "SMCs secrete the proteoglycans responsible for the initial binding and retention of atherogenic lipoproteins in the artery intima, with this retention driving foam cell formation and subsequent stages of atherosclerosis. In this chapter we review current knowledge of the extracellular matrix generated by SMCs in medial and intimal arterial layers, their relationship to atherosclerotic lesion development and stabilization, how these findings correlate with mouse models of atherosclerosis, and potential therapies aimed at targeting the SMC matrix-lipoprotein interaction for atherosclerosis prevention."

Journal • Atherosclerosis • Cardiovascular • Dyslipidemia

Tenogenic adipose-derived stem cell sheets with nanoyarn scaffolds for tendon regeneration. (PubMed, Mater Sci Eng C Mater Biol Appl) - "As shown by quantitative polymerase chain reaction and western blotting, tenogenesis-related markers (Col I&III, TNMD, biglycan, and tenascin C) were significantly increased in GDF-5-induced ASCs cell sheets compared with the uninduced...In addition, the constructs showed the potential for tendon repair in rabbit models, as demonstrated by histological, immunohistochemical, and biomechanical analyses. In our study, we successfully produced a new tissue-engineered tendon by the combination of GDF-5-induced ASCs cell sheets and nanoyarn scaffold which is valuable for tendon regeneration."

Journal • PCR

The 3D-Printed PLGA Scaffolds Loaded with Bone Marrow-Derived Mesenchymal Stem Cells Augment the Healing of Rotator Cuff Repair in the Rabbits. (PubMed, Cell Transplant) - "The mRNA expression levels of collagen I, III, tenascin, and biglycan were significantly higher in the scaffolds + BMSCs group at 4 weeks post-implantation than that in the scaffolds group...The biomechanical analysis showed that BMSCs-PLGA scaffolds implantation improved the biomechanical properties of the regenerated tendon. The combination of 3D-printed PLGA scaffolds with BMSCs can augment the tendon-bone healing in the rabbit rotator cuff repair model."

Journal • Fibrosis • PCR

Context-dependent monoclonal antibodies against protein carbamidomethyl-cysteine. (PubMed, PLoS One) - "The monoclonal antibodies label a subset of CAM-cys modified peptide sequences and purified proteins (human von Willebrand Factor (gene:vWF), Jagged 1 (gene:JAG1), Laminin subunit alpha 2 (gene:LAMA2), Thrombospondin-2 (gene:TSP2), and Collagen IV (gene:COL4)) but do not recognize specific proteins such as Bovine serum albumin (gene:BSA) and human Thrombospondin-1 (gene:TSP1), Biglycan (gene:BGN) and Decorin (gene:DCN)...Further development of novel CAM-cys monoclonal antibodies in conjunction with other biochemical tools may complement current methods for sulfhydryl detection within specific proteins. Moreover, CAM-cys reactive reagents may be useful when there is a need to label subpopulations of proteins."

Journal

Comprehensive assessment of ECM turnover using serum biomarkers establishes PBC as a high-turnover autoimmune liver disease. (PubMed, JHEP Rep) - "We assessed specific serological biomarkers of ECM turnover: type III and V collagen formation (PRO-C3, PRO-C5), degradation of type III and IV collagen (C3M, C4M), biglycan (BGM) and citrullinated vimentin (VICM)...The level of scarring is linked to prognosis in autoimmune liver diseases such as primary sclerosing cholangitis, primary biliary cholangitis, and autoimmune hepatitis; hence, the scarring process is a possible target for novel therapy. Investigating the scarring process using highly specific technology, we show that the scarring process is different between the 3 autoimmune liver diseases, and this may have important implications for the development of medical treatment."

Biomarker • Journal • Fibrosis • Gastroenterology • Gastrointestinal Disorder • Hepatology • Immunology • Inflammation • Inflammatory Bowel Disease • Liver Cirrhosis • Liver Failure • Primary Biliary Cholangitis • Ulcerative Colitis

[VIRTUAL] CIRCULATING COLLAGEN TURNOVER MARKERS ARE SPECIFICALLY CHANGED IN VERY EARLY SYSTEMIC SCLEROSIS (EULAR 2020) - "The biglycan degradation biomarker BGM was also higher in veSSc (p=0.006), whereas the degradation biomarker for type VI collagen, C6M, was lower than in HC (p=0.002)... ECM turnover is already altered in veSSc patients compared to HC. Biomarkes of type III and IV collagen distinguished between veSSc patients and HC, which may indicate them as potential biomarkers for the detection of veSSc in addition to the established immunological and capillaroscopic criteria."

Fibrosis • Scleroderma • Systemic Sclerosis

An in situ hybridization study of decorin and biglycan mRNA in mouse osteoblasts in vivo. (PubMed, Anat Sci Int) - "By contrast, decorin mRNA expression was maintained in the outer region but diminished in the interior region. These results indicate that decorin and biglycan show differential expression patterns in differentiating osteoblasts and play specific roles in bone formation."

Journal • Preclinical

[VIRTUAL] Levels of Osteopontin (SPP1), Osteonectin (SPARC) and Biglycan (BGN) in Acute Myeloid Leukemia Bone Marrow Biopsies Post-Induction Therapy Define the Status of Osteogenic Niche and Show Inverse Correlation with Therapeutic Response (ASH 2020) - "Methods : Transcriptomics and histopathologic evaluations of matched human AML core bone marrow biopsies obtained at diagnosis (n=12) and day 14 post-induction therapy (n=12) with daunorubicin and cytarabine (7+3) were performed. To our knowledge this is a first report showing the correlation between levels of osteopontin (SPP1), osteonectin (SPARC) and biglycan (BGN) and response to chemotherapy directly in the AML core bone marrow biopsies. Our data suggest that osteo-stimulatory factors could be used to achieve better therapeutic outcomes in AML."

Acute Myelogenous Leukemia • Hematological Disorders • Hematological Malignancies • Leukemia • Oncology • SPP1

Transcriptomic Profiling of Fetal Membranes of Mice Deficient in Biglycan and Decorin as a Model of Preterm Birth. (PubMed, Biol Reprod) - "Differentially expressed genes in the top three molecular pathways (1) showed evidence of negative or purifying selection, and (2) were significantly enriched (Z-score > 10) for transcription factor binding sites for Nr2f1 at E18. We propose that in DKO mice, cell cycle arrest results in lack of cell proliferation in fetal membranes, inability to contain the growing fetus, and preterm birth."

Journal • CDK1 • CDK2 • CDKN1A • NR2F1

The Inhibition of Colorectal Cancer Growth by the Natural Product Macrocarpal I. (PubMed, Free Radic Biol Med) - "Macrocarpal I is a small molecule compound that can effectively inhibit the progression of CRC. Thus, macrocarpal I is a therapeutic compound that shows promising results in the treatment of advanced CRC."

Journal • Colorectal Cancer • Gastrointestinal Cancer • Oncology • Solid Tumor • PTP4A3

Biglycan protects human neuroblastoma cells from nitric oxide-induced death by inhibiting AMPK-mTOR mediated autophagy and intracellular ROS level. (PubMed, Biotechnol Lett) - "Here, we discovered that an NO donor, sodium nitroprusside (SNP) induced autophagy in human SH-SY5Y neuroblastoma cells, including activating LC3B and inhibiting p62. Inhibiting autophagy by 3MA aggravated NO-induced cell death, otherwise promoting autophagy by Rapamycin rescued NO-triggered cell death...Moreover, BGN overexpression also diminished NO-induced the elevation of intracellular reactive oxygen species (ROS) level, but not NO content. These findings suggest that BGN protects neuroblastoma cells from NO-induced death by suppressing autophagy-dependent AMPK-mTOR signaling and intracellular ROS level."

Journal • Alzheimer's Disease • CNS Disorders • Huntington's Disease • Movement Disorders • Neuroblastoma • Oncology • Parkinson's Disease • Solid Tumor

[VIRTUAL] Klotho Improves Renal Function in Autosomal Dominant Tubulointerstitial Kidney Disease (ADTKD-UMOD) (KIDNEY WEEK 2020) - "We identified downregulation of Transforming growth factor-beta-induced protein (TGFBI) together with multiple collagens, prolargin, biglycan, and osteoglycin/mimecan in TgKl/UmodC93F/C93F vs. UmodC93F/C93F animals... Klotho improves renal outcome in ADTKD-UMOD mice by increasing urinary UMOD secretion and ameliorating renal fibrosis by downregulation of TGFBI and collagens. TgKl/UmodC93F/C93F mice show significantly lower BUN, creatinine, and cystatin C values. Funding: NIDDK Support"

Fibrosis • Immunology • Nephrology • Renal Disease • CST3 • FGF23 • TGFBI

[VIRTUAL] Augmenter of Liver Regeneration Protects Kidney from Ischemia-Reperfusion Injury via Regulation of TLR4/MAPKs Signaling Pathway (KIDNEY WEEK 2020) - "mRNA of HMGB-1, Biglycan, HAS1, HAS2 and HAS3 was detected by real-time PCR... rhALR protects kidney from I/R injury by relieving the inflammatory responses via regulation of TLR4 signaling pathway. These results suggest that ALR might be used in the development of novel immune therapy for the renal IRI rhALR protects kidney from I/R injury by regulation of TLR4 signaling pathway Funding: Government Support - Non-U. S."

IO Biomarker • Immunology • Inflammation • Reperfusion Injury • HAS 3 • MAPK8 • TLR4

Relative Protein Abundances and Biological Ageing in Whole Skeletal Elements. (PubMed, J Proteome Res) - "Most of these follow the trend of decreasing abundance through age, with the exception of prothrombin that increases. We consider the combined use of these relative abundances, along with those of previously noted fetuin-A, biglycan, albumin, and chromogranin-A signatures, as being of potential value to the development of an age estimation tool worthy of further evaluation in forensic contexts."

Journal • AHSG • APOA1 • CGA, CHGA • SPP1 • VIM

Supporting Cell-Based Tendon Therapy: Effect of PDGF-BB and Ascorbic Acid on Rabbit Achilles Tenocytes in Vitro. (PubMed, Int J Mol Sci) - "Vice versa, there was an upregulation of fibronectin, biglycan and tenascin-C by PDGF-BB supplementation, while ascorbic acid led to a downregulation of these markers. However, both biomolecules are promising candidates for improving and accelerating the in vitro expansion of tenocytes, which is vital for various tendon tissue engineering approaches or cell-based tendon therapy."

Journal • Preclinical

Bronchial Vascular Remodeling Is Attenuated by Anti-IL-17 in Asthmatic Responses Exacerbated by LPS. (PubMed, Front Pharmacol) - "The OVA-LPS-anti-IL-17 group presented a decrease in several factors [airway resistance and elastance, bronchoalveolar lavage fluid (BALF) cell counts, inflammatory response, eosinophils, TSLP, IL-33, TARC, TNF-α, CD4+, CD8+, IL-4, IL-6, IL-10, IL-17, and VEGF positive cells/10μm, peribronchovascular edema, and angiogenesis], including remodeling (MMP-9, MMP-12, TIMP-1 and TGF-β positive cells and volume fraction of collagen fibers I, collagen fibers III, collagen fibers V, decorin, lumican, actin, biglycan, fibronectin, and integrin), oxidative stress (iNOS positive cells and volume fraction of PGF2α), and signaling pathways (FoxP3), as well as dendritic cells, NF-kB, ROCK-1, ROCK-2, STAT-1, and phosphor-STAT1-positive cells compared to OVA-LPS (p < 0.05). In this model of LPS-induced asthma exacerbation, IL-17 inhibition represents a promising therapeutic strategy, indicating the potential of bronchial vascular control of Th2 and Th17 responses and the activation of..."

Journal • Asthma • Immunology • Inflammation • Respiratory Diseases • CD8 • FN1 • FOXP3 • IL10 • IL4 • IL6 • LUM • MMP9 • NFKB1 • TIMP1

Biglycan and chondroitin sulfate play pivotal roles in bone toughness via retaining bound water in bone mineral matrix. (PubMed, Matrix Biol) - "In summary, the results of this study suggest that Bgn and CS in bone matrix play a pivotal role in imparting the toughness to bone most likely via retaining bound water in bone matrix. Moreover, supplementation of CS improves the toughness of bone in mouse models."

Journal

Gene expression in urinary incontinence and pelvic organ prolapse: a review of literature. (PubMed, Curr Opin Obstet Gynecol) - "The current review highlights many genes which are potential biomarkers and targets for drug development."

Journal • Overactive Bladder • Urinary Incontinence • Urology • APOE • Collagen Type IV • CTNNB1 • ER • MMP9 • RASGRP1 • SLPI • STAT3

Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic Analysis. (PubMed, Front Oncol) - "Blocking antibodies against the proteoglycan biglycan, galectin-3 binding protein, and tissue inhibitor of metalloproteinases-2 decreased only the proliferation but not the invasion of PC cells. Together, this study delineates the secretome of human SCs and identifies proteins that can stimulate PC cell growth and invasion and therefore constitute potential therapeutic targets."

Journal • Gastrointestinal Cancer • Hepatology • Metabolic Disorders • Oncology • Pancreatic Cancer • Solid Tumor • CTSD • LGALS3BP • MMP2 • PAI1

Part 1: profiling extra cellular matrix core proteome of human fetal nucleus pulposus in search for regenerative targets. (PubMed, Sci Rep) - "Of the 33 upregulated proteins in fetus NP's, 20 of which were involved in ECM assembly pathways: fibromodulin, biglycan, heparan sulfate proteoglycan 2, chondroitin sulfate proteoglycan 4, procollagen C-endopeptidase enhancer and Collagen-type 1a1, 1a2, 6a1, 6a3, 11a1, 11a2, 12a1, 14a1 and 15a1...In conclusion, the consistency of human fetal NP's differs greatly from that of healthy adults. In view of these outcomes, the core matrisome of human fetal discs contains an abundant number of proteins that could potentially show regenerative properties, and their potential should be explored in future machinal experiments."

Journal • HSPG2 • PCOLCE • THBS1

Age-Dependent Changes of Adipokine and Cytokine Secretion From Rat Adipose Tissue by Endogenous and Exogenous Toll-Like Receptor Agonists. (PubMed, Front Immunol) - "In the present study, ex vivo fat explants were used to investigate how the exogenous pathogen-associated molecular pattern (PAMP) LPS or the endogenous danger-associated molecular patterns (DAMPs) high mobility group box-1 protein (HMGB1) and biglycan modulate the release of cytokines and adipokines/batokines and, thus, could influence systemic and/or local inflammation...Moreover, aging may increase or decrease the release of fat-derived mediators. These data show that DAMPS and LPS locally modulate cytokine secretion while only DAMPS but not LPS can locally alter adipokine secretion during inflammation."

IO Biomarker • Journal • Preclinical • Genetic Disorders • Immunology • Inflammation • Obesity • AGER • HMGB1 • IL6 • LEP • NAMPT • TLR4 • TNFA

Adding exogenous biglycan or decorin improves tendon formation for equine peritenon and tendon proper cells in vitro. (PubMed, BMC Musculoskelet Disord) - "The SLRP-supplemented peritenon cells produced constructs with greater mechanical and material properties than tendon proper seeded constructs, as well as increased expression of matrix assembly molecules. These findings provide evidence that SLRPs should be further investigated for their potential to improve tendon formation in engineered grafts or post-injury."

Journal • Preclinical

Quantitative Proteome of Infant Stenotic Ureters Reveals Extracellular Matrix Organization and Oxidative Stress Dysregulation Underlying Ureteropelvic Junction Obstruction. (PubMed, Proteomics Clin Appl) - "Significant expression of Biglycan, Fibulin-1, Myosin-10, Cytochrome b5 were validated by western blot, providing possible mechanism in UPJO which could be associated impaired smooth muscle cell, epithelial integrity and increased oxidative stress...Significant expression of Biglycan, Fibulin-1, Myosin-10, Cytochrome b5 were validated by western blot, providing possible mechanism in UPJO which could be associated impaired smooth muscle cell, epithelial integrity and increased oxidative stress. CONCLUSIONS AND CLINICAL RELEVANCE: This proteomic study provides molecular evidence of dysregulated extracellular matrix organization, impaired smooth muscle cell and oxidative stress during UPJO pathology, indicating that Biglycan, Fibulin-1, Myosin-10, Cytochrome b5 might reflect the pathology of UPJO and further studies are still needed to investigate their clinical potential."

Journal • Nephrology • CYB5A • FBLN1

Serum amyloid A-containing HDL binds adipocyte-derived versican and macrophage-derived biglycan, reducing its anti-inflammatory properties. (PubMed, JCI Insight) - "Similar to adipocytes, normal HDL exerts its anti-inflammatory function in macrophages by reducing lipid rafts, reactive oxygen species generation and translocation of toll like receptor 4 and NADPH oxidase 2 into lipid rafts, effects that are not observed with SAA-enriched HDL. These findings imply that SAA present in HDL can be trapped by adipocyte-derived versican and macrophage-derived biglycan, thereby blunting HDL's anti-inflammatory properties."

Journal • Immunology • Inflammation • Obesity • APOA1 • TLR4 • VCAN