NanO2 (perflenapent) / NuvOx Pharma - LARVOL DELTA

Reduced Nitrates/Nitrites and Natural Additives in Organic Fermented Dry-Cured Sausages: Effects on Microbiological Safety, Quality, and Consumer Acceptance. (PubMed, J Food Sci) - "Five treatments were produced: C1 (80 mg/kg KNO3), HXT100 (40 mg/kg KNO3 + 100 mg/kg HXT), HXT300 (40 mg/kg KNO3 + 300 mg/kg HXT), C2 (80 mg/kg NaNO2), and HXT200 (40 mg/kg KNO2 + 200 mg/kg HXT)...These findings may support the development of cleaner label products with fewer additives and greater health appeal. Nonetheless, consumer sensory evaluations favored traditional formulations in terms of taste, texture, and overall quality."

Journal

O. fragrans flower ameliorates compound 48/80-induced pseudo-allergic reactions in vitro and in vivo. (PubMed, Int Arch Allergy Immunol) - "This study indicates, for the first time, that OFE is a mast cell stabilizer. It decreases Ca2+[c] to suppress mast cell degranulation by directly enhancing mitochondrial Ca2+ uptake. These findings may unveil a potential medicinal value of O. fragrans flower as a promising candidate for relieving mast cell activation-related diseases."

Journal • Preclinical • Allergy • RHOD

Hybrid Oxygen-Sensing Bio-Scaffolds for 3D Micro-Tissue Models. (PubMed, Biosensors (Basel)) - "The results show superiority of the Matrigel hybrids with NanO2 and Nano-IR probes. Demonstration experiments were conducted with HCT116 cells and individual spheroids derived from these cells, culturing them in the Matrigel-NP hybrid scaffolds and monitoring oxygenation and local O2 gradients on a time-resolved fluorescence plate reader and by phosphorescence lifetime imaging microscopy (PLIM)."

Journal

Hydration and transport properties of cesium hydroxide and mixed cesium hydroxide-sodium nitrite aqueous solutions. (PubMed, J Chem Phys) - "This study explores the hydration and transport properties of aqueous cesium hydroxide (CsOH) solution, with or without 1 molar (M) sodium nitrite (NaNO2)...Nonetheless, ion-pairing or obstructions did result in more sluggish transport properties as electrolyte concentrations increased. Overall, the research enhances the understanding of the complexities underlying ion interactions in multicomponent solutions."

Journal

EXTEND-1b: A Dose Escalation Study of NanO2 in Patients With Mild Respiratory Distress (clinicaltrials.gov) - P1 | N=18 | Recruiting | Sponsor: NuvOx LLC | Trial completion date: Dec 2025 ➔ Dec 2026 | Trial primary completion date: Dec 2025 ➔ Dec 2026

Trial completion date • Trial primary completion date

Sodium nitrite impairs reproduction in female mice by disrupting ovarian steroid hormone synthesis and lipid metabolic pathways. (PubMed, Ecotoxicol Environ Saf) - "Integrated transcriptomic and metabolomic analyses revealed abnormalities in steroid hormone synthesis and lipid metabolism pathways in the ovaries. We found that NaNO2 exerts detrimental effects on female reproductive ability in mice by disrupting the synthesis of ovarian steroid hormones and lipid metabolism."

Journal • Preclinical • Dyslipidemia • Metabolic Disorders

Direct diazotization of indoles with 2-Methoxyethyl nitrite. (PubMed, Commun Chem) - "MOE-ONO showed higher reactivity and selectivity than conventional NO donors, even including tert-butyl nitrite or NaNO2/AcOH...The synthesized 3-diazoindoles exhibit broad reactivity, such as Grignard reaction at the C2-position and rhodium-catalyzed cyclopropanation at the C3-position. Such reactions can be practical solutions for synthesizing highly functionalized indoles and indolines, which are important for the design and discovery of pharmacologically active compounds."

Journal

Integrated metabolomics and volatilomics reveal the impact of compound curing agents on lipid-related metabolites and volatile compounds in Xuanwei ham. (PubMed, Food Res Int) - "To address these issues, this study investigated the effects of compound curing agents, specifically partial replacement of NaCl with KCl and NaNO2, with or without added sucrose, on the subcutaneous fat of Xuanwei ham...Correlation analysis revealed that the distinct aroma profile was associated with the curing agents' modulation of lipid β-oxidation, amino acid metabolism, and enhanced microbial ester synthesis. These findings provide a scientific basis for the development of low-sodium hams with stable color and optimized flavor."

Journal • Metabolic Disorders

Characterization of Poly(vinyl alcohol)/Carboxymethyl Cellulose Blends with NaNO2 to Develop Active Packaging for Use with Meat. (PubMed, ACS Omega) - "Taken together, the results indicate that PVA/CMC blends have potential as active films for meat applications, offering good mechanical properties and nitrite release capacity. However, optimizations are needed to improve the moisture barrier and antioxidant efficacy in the product."

Journal

Chelesta-8,24-dien-3-ol in Ficus exasperata leaves enhances the prevention of sodium nitrite-induced hypoxia by binding to HIF-1 and NF-κB. (PubMed, Sci Rep) - "Cholesta-8,24-dien-3-ol, 4-methyl- (3β), has the highest binding affinity for HIF-1 (ΔG = -8.6 kcal/mol) and NF-κB (ΔG = -7.6 kcal/mol) proteins among the NHEAF phytochemicals. Cholesta-8,24-dien-3-ol, 4-methyl-, (3β) may contribute to NHEAF's protection against s NaNO2-induced hypoxic stress."

Journal • HIF1A

Acrylic acid functionalized silica composite resin for selective recovery of Ru from simulated high-level liquid waste. (PubMed, Environ Res) - "The resin maintained stable reusability over 5 cycles using 0.5 M NaNO2 as eluent with only 3.77 mg Ru/g decrease in adsorption capacity. This work provides a cost-effective, high selective, and robust resin for sustainable Ru recovery from HLLW."

Journal

A novel combined use of FeCl3 and NaNO2 markedly improves the efficiency of aerobic waste activated sludge treatment. (PubMed, Water Res) - "The dosed iron also captured phosphate released during digestion, which was subsequently transformed into vivianite in an anoxic bioprocess and successfully separated from sludge through acidic leaching. Overall, the integrated process achieved simultaneous sludge minimization, quality enhancement, and resource recovery, highlighting the potential of ferric salt-assisted pretreatment as a sustainable WAS management strategy within a circular economy framework."

Journal

Improved Storage Stability of Sausage Based on the Dual Effects of Sodium Nitrite on Color Development and Bacterial Inhibition. (PubMed, J Food Sci) - "PRACTICAL APPLICATIONS: This study provides a practical strategy for reducing the total amount of NaNO2 used in sausages without compromising product quality. Distributing NaNO2 between the meat batter and the casing effectively maintained color, enhanced microbial safety, and preserved the antioxidant capacity of sausages."

Journal • MB

Influences of three natural antioxidants on the quality of Chinese-style traditional sausage during storage. (PubMed, J Sci Food Agric) - "In processed meat products, TF-NaNO₂ complexes acted as an effective partial nitrite replacer by providing synergistic antioxidant and antimicrobial effects."

Journal

Immunomodulation of Tumor Microenvironment by Reversing Hypoxia (SABCS 2025) - "NanO₂ (NuvOx Pharma), a novel perfluorocarbon-based oxygen therapeutic composed of 2% w/v dodecafluoropentane, improves tissue oxygenation without receptor binding...In the second series of experiments, we tested the effect of NanO₂ in combination with immunotherapy (IT), chemotherapy (CTX, paclitaxel) or both IT and CTX...NanO₂ synergizes with immunotherapy and chemotherapy to remodel the TME to enhance anti-tumor immune responses in in vivo TNBC models.Figures to Upload:CD8 IHC Decreased Hypoxic Cells"

Biomarker • Immunomodulating • IO biomarker • Tumor microenvironment • Breast Cancer • Oncology • Triple Negative Breast Cancer • CD4 • CD8 • MRC1

Visible-light promoted late-stage chlorination and bromination of quinones and (hetero)arenes utilizing aqueous HCl or HBr as halogen donors. (PubMed, Commun Chem) - "The mild reaction conditions, ready availability of reagents, excellent functional-group tolerance, high regioselectivity, and facile scalability under continuous-flow operation collectively render this approach a practical and efficient protocol for the late-stage aromatic C(sp²)-H chlorination and bromination of complex drugs and natural products. Mechanistic investigations reveal that nitrosyl halides, generated in situ by reaction of NaNO2 and aqueous HCl or HBr, undergo photo-promoted homolysis to produce the corresponding halogen radicals that selectively initiate the radical halogenation of quinones and (hetero)arenes."

Journal

Rising trends in sodium nitrite suicides: A case series from Cape Town, South Africa. (PubMed, S Afr Med J) - "Sodium nitrite (NaNO2) ingestion results in methaemoglobinaemia, which can cause hypoxia, metabolic acidosis and death...This report highlights the importance of thorough death scene investigations and challenges in ancillary testing, and contributes to the literature on sodium nitrite-related suicides. The accessibility and rapid lethality of the chemical underscore the need for greater awareness among medical professionals and policy-makers regarding its misuse."

Journal • Metabolic Disorders

Visible-Light-Induced Trifluoromethyloximation of Alkenes with Sodium Nitrite as Oxime Source. (PubMed, J Org Chem) - "The strategy employs commercially abundant and robust alkenes as starting materials, economical CF3SO2Cl as the trifluoromethylating agent, and NaNO2 as the nitrosyl source, operating without transition-metal catalysts or external photosensitizers. This approach demonstrates broad functional group compatibility, high selectivity, mild reaction conditions, and excellent practicality."

Journal

A phase 2 double-blind, randomized, prospective, placebo-controlled study of NanO₂TM combined with radiation and temozolomide in patients with newly diagnosed glioblastoma: RESTORE (SNO 2025) - "This study hypothesizes that increasing oxygen delivery using NanO₂ (2% w/v dodecafluoropentane emulsion) infusion and inhaled oxygen will enhance the efficacy of chemoradiation. This ongoing randomized trial is assessing the safety and potential survival benefit of NanO₂ in newly diagnosed GBM. By improving oxygen delivery to hypoxic tumor regions, NanO₂ may enhance the efficacy of chemoradiation. The study remains blinded, and no preliminary efficacy conclusions can be made at this time."

Clinical • P2 data • Brain Cancer • Cardiovascular • CNS Disorders • Epilepsy • Glioblastoma • Hematological Disorders • Pulmonary Embolism • Respiratory Diseases • Solid Tumor • Thrombocytopenia

Spontaneous Graphene/Lipid Bicellar Co-assembly (NANO2-Graphene): Experiments and Computer Simulations. (PubMed, ACS Appl Mater Interfaces) - "MD simulations also reveal that, in the presence of single or stacked graphene layers, lipid tails bend and orient along the graphene surface to maintain the bilayer thickness with the lipid order parameter significantly reduced and the area per lipid increased along with water contacts with lipid headgroups. These results provide important insights into the interaction between lipid molecules and graphene that should be considered in the biomedical application of graphene-containing nanomaterials and, more broadly, the effect of graphene on lipid-containing biointerfaces."

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A phase 2 double-blind, randomized, prospective, placebo-controlled study of  NanO₂TM combined with radiation and temozolomide in patients with newly diagnosed glioblastoma: RESTORE (WFNOS 2025) - "This study hypothesizes that increasing oxygen delivery using NanO₂ (2% w/v dodecafluoropentane emulsion) infusion and inhaled oxygen will enhance the efficacy of chemoradiation. This ongoing randomized trial is assessing the safety and potential survival benefit of NanO₂ in newly diagnosed GBM. By improving oxygen delivery to hypoxic tumor regions, NanO₂ may enhance the efficacy of chemoradiation. The study remains blinded, and no preliminary efficacy conclusions can be made at this time."

Clinical • P2 data • Brain Cancer • Cardiovascular • CNS Disorders • Epilepsy • Glioblastoma • Glioma • Hematological Disorders • Oncology • Pulmonary Embolism • Respiratory Diseases • Solid Tumor • Thrombocytopenia

FYR Bio and NuvOx Therapeutics Awarded NCI Direct-to-Phase II SBIR Grant to Advance Liquid-Biopsy Collaboration in Phase IIb Glioblastoma Trial (PRNewswire) - "Under the award, FYR will apply its EV-Omics (EVO) platform and SPARCs enrichment technology to longitudinal blood samples collected in NuvOx's RESTORE trial of NanO₂, a radiosensitizer designed to reverse tumor hypoxia. The collaboration will explore extracellular-vesicle (EV) biomarkers that reflect hypoxia biology, correlate with treatment exposure, and help clinicians differentiate pseudoprogression from true progression-a persistent challenge in GBM management."

Financing • Glioblastoma

Investigation of the use of anthocyanins in Cantonese sausage as a nitrite alternative. (PubMed, J Sci Food Agric) - "Cantonese sausages treated with anthocyanins exhibited benefits in TBARS value, residual nitrite levels, and color, and did not compromise antibacterial efficacy. This study demonstrated the possibility of anthocyanins (C3G) as a natural nutrient to partially replace nitrite."

Journal • Oncology

The Effect of Swiss Chard Powder as a Curing Agent on Volatile Compound Profile and Other Qualitative Properties of Heat-Treated Sucuk. (PubMed, Foods) - "Three formulations were created for the production of HTS: control (no nitrite addition), synthetic nitrite (SN, 150 mg/kg NaNO2 addition), and natural nitrite from Swiss chard powder (SCPN) (SCP equivalent to 150 mg/kg NaNO2)...Additionally, similar characteristics in volatile compounds and sensory attributes were observed in the SN and SCPN groups. As a result, characteristics of the final products were not usually adversely affected by the use of SCP in HTS production."

Journal

Immunomodulation of Tumor Microenvironment in Triple Negative Breast Cancer by Reversing Hypoxia with NanO2, a Novel Oxygen Delivery Agent (SITC 2025) - "In the second series of experiments, we tested the effect of NanO2 in combination with immunotherapy (IT), chemotherapy (CTX, paclitaxel) or both IT and CTX. PD-1 expression decreased supporting that reversing hypoxia can enhance response to IT. In addition, in the second series of experiments we demonstrated that the TME changes by NanO2 +oxygen were enhanced by IT, CTX and combination IT+CTX (figure 2).Conclusions NanO2 synergizes with immunotherapy and chemotherapy to remodel the TME to enhance anti-tumor immune responses in in vivo TNBC models.Acknowledgements This study was funded by DOD Grant to Pavani Chalasani (Award number:W81XWH2210640Ethics Approval Study was approved by IACUC at GWUC and DOD ACCUROAbstracts 5 Figure 1Request permissionsCD8 IHC resultsAbstracts 5 Figure 2Request permissionsHypoxia cells by flow cytometry"

Biomarker • Immunomodulating • IO biomarker • Tumor microenvironment • Brain Cancer • Breast Cancer • Glioblastoma • Oncology • Solid Tumor • Triple Negative Breast Cancer • CD4 • CD8 • MRC1