SER140 / Serodus - LARVOL DELTA

A novel short-chain dehydrogenase/reductase catalyzing efficient patulin detoxification: identification and computational characterization. (PubMed, J Sci Food Agric) - "The acidic pH preference of LmSDR indicates its optimal activity under mildly acidic conditions, highlighting its potential as a biocatalyst for applications in acidic food matrices. Moreover, computer-aided approaches offer a theoretical framework for elucidating the proton transfer mechanism of LmSDR and other PAT-degrading SDRs."

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Molecular insights into the catalytic mechanism of a phthalate ester hydrolase. (PubMed, J Hazard Mater) - "The catalytic mechanism of HylD1 mediated by the catalytic triad Ser140-Asp231-His261 was further proposed. The hylD1 gene is widely distributed in different environments, suggesting its important role in PAEs degradation. This study provides a better understanding of PAEs hydrolysis, and lays out favorable bases for the rational design of highly-efficient PAEs degradation enzymes for industrial applications in future."

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Elevated N1-Acetylspermidine Levels in Doxorubicin-treated MCF-7 Cancer Cells: Histone Deacetylase 10 Inhibition with an N1-Acetylspermidine Mimetic. (PubMed, J Cancer Prev) - "MINAS and Tubastatin A share similar binding sites on HDAC10, including Ser138, Ser140, Tyr183, and Cys184. In conclusion, the data show that N1-acetylspermidine levels rise during DOX-induced breast cancer cell death. Additionally, MINAS, an N1-acetylspermidine mimetic compound, could be investigated as a potential anticancer drug when combined with chemotherapy like DOX."

Epigenetic controller • Journal • Breast Cancer • Oncology • Solid Tumor • HDAC1 • HDAC10

Origin of Chemoselectivity of Halohydrin Dehalogenase-Catalyzed Epoxide Ring-Opening Reactions. (PubMed, J Chem Inf Model) - "A hydrogen bonding network formed by residues Ser140, Tyr153, and Arg157 can strengthen the electrophilicity of the active site of the epoxide substrate to affect chemoselectivity. To predict the energy barrier trends of the chemoselective transition states, multiple analyses including distortion analysis and electrophilic Parr function (Pk+) analysis were carried out with or without an enzyme environment. The obtained insights should be valuable for the rational design of enzyme-catalyzed and biomimetic organocatalytic epoxide ring-opening reactions with special chemoselectivity."

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Molecular cloning and biochemical characterization of a NAD-dependent sorbitol dehydrogenase from cold-adapted Pseudomonas mandelii. (PubMed, FEMS Microbiol Lett) - "PmSDH contained Asn111, Ser140, Tyr153, and Lys157 as catalytic active site residues and existed as a ∼67 kDa dimer in size-exclusion chromatography...PmSDH maintained its conformational flexibility, secondary and tertiary structures, and thermal stability at 4-25°C. At 40°C, PmSDH was rapidly denatured. These results indicate that PmSDH, which has a flexible structure and a high catalytic activity at colder temperatures, is well-suited to sorbitol utilization in the cold-adapted bacterium P. mandelii JR-1."

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Biodegradation of phthalic acid esters (PAEs) by Cupriavidus oxalaticus strain E3 isolated from sediment and characterization of monoester hydrolases. (PubMed, Chemosphere) - "The docking results showed that the conserved catalytic triplet structure (Ser140, His284, and Asp254) acted as active sites and participated in degrading PMEs. This study provided novel insights into the mechanisms of PAEs degradation at a molecular level and widened the scope of functional bacteria by isolating strain E3."

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FBW7 inhibits myeloid differentiation in acute myeloid leukemia via GSK3-dependent ubiquitination of PU.1. (PubMed, Mol Cancer Res) - "We demonstrate that GSK3β phosphorylates PU.1 at Ser41 and Ser140 leading to its recognition and subsequent ubiquitin-mediated degradation by E3 ubiquitin ligase FBW7...In addition, PBMCs also showed enhanced differentiation when treated with M-CSF and GSK3 inhibitor (SB216763) together compared to M-CSF treatment alone. Implications: Our data demonstrate a plausible mechanism behind PU.1 restoration and induction of myeloid differentiation upon GSK3β inhibition and further substantiates potential of GSK3β as a therapeutic target in AML."

Journal • Acute Myelogenous Leukemia • Hematological Malignancies • Leukemia • Oncology • Targeted Protein Degradation • CSF1 • FBXW7 • SPI1

Characterization of an extracellular protein, Rv1076 from M. tuberculosis with a potential role in humoral response. (PubMed) - Int J Biol Macromol - "Rv1076 elicited strong humoral response in both extrapulmonary and relapsed cases of TB patients. Therefore, we conclude that Rv1076 is a novel secretory esterase of M. tuberculosis which could be a potential immunodominant antigen of M. tuberculosis."

Journal • Biosimilar

Characterization and function of Mycobacterium tuberculosis H37Rv Lipase Rv1076 (LipU). (PubMed) - Microbiol Res - "The active-site residues of LipU were determined to be Ser140, Asp244 and His269 by site-directed mutagenesis. The upregulation of Mycobacterium tuberculosis rv1076 under nutritive stress implicates a role in starvation."

Journal • Biosimilar

WIP1 dephosphorylation of p27 Serine 140 destabilizes p27 and reverses anti-proliferative effects of ATM phosphorylation. (PubMed, Cell Cycle) - "We demonstrate that wildtype, but not phosphatase-dead WIP1, efficiently dephosphorylates p27 Ser140 both in vitro and in cells and that this dephosphorylation is sensitive to the WIP1-specific inhibitor GSK 2830371...Overexpression of wildtype p27 reduces cell proliferation and colony forming capability relative to the S140A (constitutively non-phosphorylated) form of p27. Thus, WIP1 plays a significant role in homeostatic modulation of p27 activity following activation by ATM."

Journal • ATR • CDKN1B

A signalling cascade involving receptor-activated phospholipase A, glycerophosphoinositol 4-phosphate, Shp1 and Src in the activation of cell motility. (PubMed, Cell Commun Signal) - "This study identifies a so-far undescribed mechanism of Shp1/Src modulation that promotes cell motility and that is dependent on the cPLA metabolite GroPIns4P. We show that GroPIns4P is required for EGF-induced fibroblast migration and that it is part of a cPLA/GroPIns4P/Shp1/Src cascade that might have broad implications for studies of immune-inflammatory response and cancer."

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