IL-15 DC / Baylor University - LARVOL DELTA

Autophagy in antigen presenting cells enhances the adjuvant effect by promoting antigen processing. (IMMUNOLOGY 2022) - "Contrary to active antigen processing, GM-CSF and IL-4 - DC did not show inducible effect after adjuvant treatment, but GM-CSF or GM-CSF and IL-15-DC increased autophagy function after adjuvant treatment that was assessed by using fluorescent labeled phagosome or lysosome function analysis. Additionally, we investigated gene expression pattern related to autophagy such as ATG5 or ATG18 by q-PCR, and western blotting. Cultured DC with GM-CSF and IL-15 indicated prominent active autophagy related gene expression pattern.  To induce more effective immune response on vaccination or immune therapy, effect of adjuvant treatment for autophagy function in DC and macrophage are required."

Clinical • Targeted Protein Degradation • CD8 • CSF2 • IFNG • IL15 • IL4 • WIPI1

Anti-Tumor Potency of Short-Term Interleukin-15 Dendritic Cells Is Potentiated by In Situ Silencing of Programmed-Death Ligands. (PubMed, Front Immunol) - "Ultimately designed to induce a durable tumor antigen-specific immune response, PD-L silenced IL-15 DCs were capable of surpassing PD-1-mediated inhibition by antigen-specific T cells. Further corroborating the superior potency of short-term IL-15 DCs, the combination of immune stimulatory components during DC differentiation and maturation with in situ checkpoint inhibition supports further clinical translation."

Journal • Immune Modulation • Immunology • Inflammation • Oncology • Solid Tumor • Wilms Tumor • IFNG • IL15 • PD-L1 • PD-L2

IL15 Dendritic Cell Vaccine for Patients With Resected Stage III (A, B or C) or Stage IV Melanoma (clinicaltrials.gov) - P1/2; N=20; Active, not recruiting; Sponsor: Baylor Research Institute; Trial primary completion date: Dec 2015 ➔ Dec 2016

Trial primary completion date • Biosimilar • Melanoma • Oncology

CD56 Homodimerization and Participation in Anti-Tumor Immune Effector Cell Functioning: A Role for Interleukin-15. (PubMed, Cancers (Basel)) - "...Next, through neutralization of surface CD56, we were able to (1) demonstrate the direct involvement of CD56 in tumor cell lysis exerted by CD56-expressing killer cells, such as natural killer cells, gamma delta (γδ) T cells, and interleukin (IL)-15-cultured dendritic cells (DCs), and (2) reveal a putative crosstalk mechanism between IL-15 DCs and CD8 T cells, suggesting CD56 as a co-stimulatory molecule in their cell-to-cell contact...Finally, by blocking the mitogen-activated protein kinase (MAPK) pathway and the phosphoinositide 3-kinase (PI3K)-Akt pathway, we showed that IL-15 stimulation directly led to CD56 upregulation. In conclusion, these results underscore the previously neglected importance of CD56 expression on immune cells, benefiting current and future immune therapeutic options."

Journal

Antigen processing and autophagy function in adjuvant treated dendritic cell. (AAI 2019) - "...Contrary to active antigen processing, GM-CSF with IL-14 –DC did not show any inducible effect after adjuvant treatment, but GM-CSF or GM-CSF with IL-15-DC increased the autophagy function. On the other hand, macrophage could not obtain autophagy effect thorough adjuvant treatment. DCs were studied the gene expression of cytokines, cell surface molecules such as co-stimulation molecules, mannose receptor, to evaluate antigen uptake capacity, processing pattern and autophagy"

Clinical