CS-2100 / Daiichi Sankyo - LARVOL DELTA

Presynaptic Release-Regulating Sphingosine 1-Phosphate 1/3 Receptors in Cortical Glutamatergic Terminals: Adaptations in EAE Mice and Impact of Therapeutic FTY720. (PubMed, Cells) - "The conclusion relies on the findings that (i) the S1P1R agonist CS-2100 (0.1-30 nM) inhibits the 12 mM KCl-evoked glutamate exocytosis (quantified as the release of [H]D-aspartate) while the S1P3R allosteric agonist CYM-5541 potentiates it and (ii) these effects are inhibited by the S1P1R antagonist Ex 26 (30-300 nM) and the S1P3R antagonist TY-52156 (100-1000 nM), respectively. These results improve our knowledge of the role of presynaptic release-regulating S1P1Rs and S1P3Rs controlling glutamate transmission in the CNS also unravelling functional adaptations during EAE that recover following chronic FTY720. In a whole, these findings provide new information on the central neuroprotectant activities of FTY720."

Journal • Preclinical • CNS Disorders • Immunology • Multiple Sclerosis • S1PR1

Routine Measurement of Direct Factor Xa Inhibiting Anticoagulants (ISTH 2022) - "Background: The licensing of andexanet alfa for the reversal of rivaroxaban in bleeding patients with levels above 50ng/mL means direct factor Xa inhibitor (DFXaI) assays may be urgently required... HB-LRT Heparin reagents were used with the standard protocols and the combined protocol, which uses a 1/15 sample dilution.Samples were measured on a Sysmex CS-2100 using standard protocols, and on Sysmex CS-5100 and CS-2500 analysers using the combined protocol.Hyphen-Biomed Rivaroxaban Controls 1 and 2 were tested five times a day for five days using the standard protocols for apixaban and edoxaban, to derive target values for use in the combined assay... Using drug-specific calibrators in the combined protocol, the difference in optical density between highest and lowest calibrators was not significantly different from those in dedicated rivaroxaban or apixaban assays.Standard and combined protocols showed good correlation for patients samples.Standard and combined..."

Precision of One-Stage Clotting Factor Assays is Improved by Using Fresh Rather Than Stored Calibration Curves (ISTH 2022) - "Aims: We evaluated the imprecision of the control results for all one-stage clotting factor assays (OSCA) performed on one Sysmex CS-2100 analyser for four years using stored curves and four years using fresh curves... For PT-based OSCA, the mean CV for stored curves was 8.21% for normal controls and 8.8% for pathological controls; for fresh curves the CVs were 4.87% and 5.00% respectively. For APTT-based OSCA the values were 7.23% and 8.75% for stored curves and 5.49% and 5.45% for fresh curves. These differences are statistically significant (see Table 1)."

Hematological Disorders

Determining the maximal storage time of centrifuged citrated samples for performing add-on routine coagulation tests. (PubMed, Thromb Res) - "Analytical evaluation of routine coagulation tests resulted in various significant differences and large variations between the various time intervals. Our results indicated that the PT and INR can be measured up till 24 h of storage. Centrifuged blood for measuring the fibrinogen concentration, antithrombin activity, D-dimer concentration and thrombin time can be stored up to 4 h, while 2 h of storage might already be too long for obtaining reliable aPTT measurements."

Journal • F2

How Do Contrast Agents Affect Cardiac Markers and Coagulation Tests? Experimental Study. (PubMed, Comb Chem High Throughput Screen) - "Different contrast media may cause false negatives in cTnI and Nt-proBNP. The contrast medium that causes the least interference should be preferred. The results of samples taken in the first hour after contrast imaging should be interpreted with care."

Journal

THE IMPACT OF SPECIMEN VOLUME AND HAEMOLYSIS ON ROUTINE COAGULATION TEST RESULTS: REDUCING THE RECOLLECTION BURDEN IN A HIGH-THROUGHPUT LABORATORY (EHA 2019) - "...All testing was performed using photo-optical measurements on a Sysmex® CS-2100 i coagulation analyser...Evidence-based acceptance criteria was developed using these findings resulting in significant cost savings, improved turnaround times and increased satisfaction across clinical and allied health teams. This review offers a clinically appropriate solution to a problem which has always hindered routine coagulation testing."