Phosribbon (monobasic sodium phosphate monohydrate/dibasic sodium phosphate anhydrous) / Zeria Pharma - LARVOL DELTA

An ultrasensitive UHPLC-ESI-MS/MS method augmented with a controlled microwave derivatization reaction for quantitation of vitamin D3 and its major metabolites in COVID-19 patients. (PubMed, Talanta) - "The precursor to product ion transitions for D3, 25-hydroxy D3 (25(OH)D3), 1,25-dihydroxy D3 (1,25-(OH)2D3) and calcipotriol (CPT) as an internal standard were m/z 493.4 → 231.3, m/z 509.4 → 231.3, m/z 525.4 → 247.3, and m/z 521.4 → 247.3; respectively...The developed method has demonstrated excellent comparability to a well-established chemiluminescent immunoassay (CLIA) method for the analysis of D3 metabolites in human samples. The developed UHPLC-ESI-MS/MS method was implemented for routine and reliable quantitation of D3 and its major metabolites in COVID-19 patients."

Journal • Infectious Disease • Novel Coronavirus Disease

Rapid screening for natural lipase inhibitors from Alisma orientale combining high-performance thin-layer chromatography-bioautography with mass spectrometry. (PubMed, J Chromatogr B Analyt Technol Biomed Life Sci) - "As a result, eleven natural lipase inhibitors from Alisma orientale extracts were identified based on molecular mass and fragment ions obtained by HPTLC-MS, and further confirmed by a series of complementary means including UV spectra, H NMR characteristic proton signals and polarity of compounds, eleven lipase inhibitors were tentatively assigned as triterpenoids: alisol B (m/z 495.50 [M + Na]), alisol B 23-acetate (m/z 537.58 [M + Na]), 11-deoxy-alisol B (m/z 479.50 [M + Na]), 11-deoxy-alisol B 23-acetate (m/z 521.50 [M + Na]), alisol A/epialisol A (m/z 513.50 [M + Na]), 16-oxo-11-deoxy-alisol A (m/z 511.50 [M + Na]), 16-oxo-alisol A (527.50 [M + Na] ), alisol C (m/z 509.58 [M + Na]), alisol C 23-acetate (m/z 551.50 [M + Na]), alisol M 23-acetate (m/z 567.50 [M + Na]), and alismanol Q/neoalisol (m/z 493.42 [M + Na]). The integrated approach is an efficient..."

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Simultaneous Determination of Ticagrelor and Its Metabolites in Human Plasma and Urine Using Liquid Chromatography-Tandem Mass Spectrometry. (PubMed, J Anal Toxicol) - "The ticagrelor and metabolite M8 (AR-C124910XX) responses were optimized at the m/z 521.2 → 361.2 and m/z 477.2 → 361.1 transitions, respectively. The relative quantification of other metabolites was performed by assessing the ratio of metabolite to IS peaks. The newly developed method was successfully used in a pharmacokinetic study characterizing ticagrelor metabolism in human volunteers."

Journal • Biosimilar

Simultaneous quantification of ticagrelor and its active metabolite, AR-C124910XX, in human plasma by liquid chromatography-tandem mass spectrometry: Applications in steady-state pharmacokinetics in patients. (PubMed, Transl Clin Pharmacol) - "Mass spectrometric detection and quantitation were conducted by selected reaction-monitoring on a negative electrospray ionization mode with the following transitions: m/z 521.11 → 361.10, 477.03 → 361.10, and 269.00 → 169.60 for ticagrelor, AR-C124910XX, and tolbutamide, respectively...Also, the anticoagulant effect was tested by lithium heparin, sodium heparin, and K-EDTA. No relevant anticoagulant effect was observed. This validated method was effectively used in the determination of ticagrelor and its active metabolite, AR-C124910XX, in plasma samples from patients with myocardial infarction."

Clinical • Journal • PK/PD data

Anaerobacillus alkaliphilus sp. nov., a novel alkaliphilic and moderately halophilic bacterium. (PubMed, Int J Syst Evol Microbiol) - "...The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10 and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006 (98.7 and 99.1 %), A. macyae JMM-4 (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6 (97.7 and 98.4 %), A. alkalilacustris Z-0521 (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340 (97.5 and 98.2 %)...is proposed. The type strain is B16-10 (=DSM 29790=NCAIM B 02608)."

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