MGN-9103 / Viridian Therap - LARVOL DELTA

[VIRTUAL] Augmentation of Cardiac Micrornas Enhanced Cardiac Mesenchymal Cell Differentiation Into Cardiomyocyte Like Cells (AHA 2021) - "The CMCs were transiently transfected with cardiac miRNAs cocktail (miR-1, miR-133, miR-208 and miR-499) and cultured for 48h... Our results demonstrate that enrichment of cardiac miRNAs in CMCs increase their differentiation into cardiomyocyte lineage, which could improve their therapeutic efficacy. Further in vivo experiments would reveal functional benefits from these improved CMCs for clinical use."

Cardiovascular • Myocardial Infarction • MEF2C • THY1

Bioengineering Methods in MicroRNA-Mediated Direct Reprogramming of Fibroblasts Into Cardiomyocytes. (PubMed, Front Cardiovasc Med) - "Among available DR methods, non-viral transfection with microRNAs (miRcombo: miR-1, miR-133, miR-208, and miR-499) appears promising for future clinical translation...Epigenetic regulation of gene expression programs, critical to cell lineage commitment, can also be promoted by the administration of specific anti-inflammatory and anti-fibrotic soluble factors, helping in suppressing fibroblast signature. The aim of this mini-review is to introduce the readers to a relatively unknown field of cardiac research integrating bioengineering tools as relevant for the progress of miRNA-mediated cardiac DR."

Journal • Review • Cardiovascular • Congestive Heart Failure • Coronary Artery Disease • Fibrosis • Heart Failure • Myocardial Infarction • Transplantation

Evaluation of Oxidative Stress, Apoptosis, and Expression of MicroRNA-208a and MicroRNA-1 in Cardiovascular Patients. (PubMed, Rep Biochem Mol Biol) - "The results showed increases in oxidative stress, inflammation, apoptotic factors, and in the expression of miR-208a in a variety of cardiovascular patients (ACS, MI, arrhythmia, and HF). It is suggested that future studies determine the relationships that miR-1, miR-208, and oxidative stress indices have with inflammation and apoptosis."

Clinical • IO biomarker • Journal • Acute Coronary Syndrome • Atrial Fibrillation • Cardiovascular • Congestive Heart Failure • Heart Failure • Immunology • Inflammation • Myocardial Infarction • BAX • BCL2 • IL10 • NOS3 • TNFA

Diagnostic accuracy of MicroRNA 208b level with respect to different types of atrial fibrillation. (PubMed, Indian Heart J) - "There was a statistically significant change of miR-208 b levels in paroxysmal (p = 0.044) and persistent (p = 0.040) AFib patients, but not for embolic CVA patients. miR-208 b could serve as a new serum marker for paroxysmal AFib."

Journal • Atrial Fibrillation • Cardiovascular • Myocardial Infarction

[VIRTUAL] Ginsenoside Rb1 and Metformin improves erythropoiesis in models of Diamond Blackfan Anemia by targeting Nemo-like Kinase (ISEH 2021) - "Metformin induces miR-26a while ginsenoside Rb1 upregulates miR-208. Both compounds significantly improve erythropoiesis in DBA models (p=0.004 and 0.008). Our results suggest that Metformin and ginseng improve erythropoiesis in DBA models and are well tolerated and widely used, offering a promising treatment for DBA patients."

Anemia • Aplastic Anemia • Diabetes • Genetic Disorders • Hematological Disorders • Metabolic Disorders • Oncology • Type 2 Diabetes Mellitus

Exploring diagnostic and prognostic predictive values of microRNAs for acute myocardial infarction: A PRISMA-compliant systematic review and meta-analysis. (PubMed, Medicine (Baltimore)) - "Circulating miR-1, miR-133, miR-208, and miR-499 showed diagnostic values in AMI."

Journal • Retrospective data • Review • Cardiovascular • Myocardial Infarction

The active component of Ginseng, Ginsenoside Rb1, improves erythropoiesis in models of Diamond Blackfan Anemia by targeting Nemo-like Kinase. (PubMed, J Biol Chem) - "We also compare ginsenoside Rb1-mediated upregulation of miR-208 with metformin-mediated upregulation of miR-26. We conclude that targeting NLK expression through miRNA binding of the unique 3'-UTR is a viable alternative to the challenges of developing small molecule inhibitors to target the highly conserved kinase domain of this specific kinase."

Journal • Anemia • Genetic Disorders • Hematological Disorders • MYB

CHOP Increases TRIB3-Dependent miR-208 Expression to Potentiate Vascular Smooth Muscle Cell Proliferation and Migration by Downregulating TIMP3 in Atherosclerosis. (PubMed, Cardiovasc Drugs Ther) - "Taken together, inhibition of CHOP may inhibit the proliferation and migration of VSMCs as well as reduce the levels of TC, TG, and LDL-C but increase the level of HDL-C through the TRIB3/miR-208/TIMP3 axis, thereby inhibiting the progression of atherosclerosis."

Journal • Atherosclerosis • Cardiovascular • Dyslipidemia • APOE • TRIB3

Ambient air PM exposure induces heart injury and cardiac hypertrophy in rats through regulation of miR-208a/b, α/β-MHC, and GATA4. (PubMed, Environ Toxicol Pharmacol) - "In this study, we investigated the miR-208/GATA4/myosin heavy chain (MHC) regulation mechanisms on cardiac injury in rats after PM exposure via an animal inhalation device...The ratios of β-MHC/α-MHC expression induced by PM were remarkably raised in comparison to their controls. It suggested that the up-regulation of miR-208b/β-MHC and GATA4 and the conversion from α-MHC to β-MHC may be the important causes of cardiac hypertrophy in rats incurred by PM."

Journal • Preclinical • Cardiovascular • Inflammation

Genome-wide identification and functional characterization of natural antisense transcripts in Salvia miltiorrhiza. (PubMed, Sci Rep) - "And we found two miRNAs including miR156a and miR7208, might originate from NATs, NAT0112 and NAT0086. The results suggest that S. miltiorrhiza NATs might interact with STs, produce miRNAs, and be regulated by miRNAs. They potentially play significant regulatory roles in the biosynthesis of bioactive compounds."

Journal

Difference in microRNA levels in the post-mortem blood from different sampling sites: A proof of concept. (PubMed, J Forensic Leg Med) - "The selected target microRNAs were cardiac-specific (miR208 and miR-1) and non-cardiac microRNAs (miR-16 and let-7e)...The present study confirms that the cardiac-specific microRNA levels in the PM blood from different sampling sites are different. In addition, this study showed that the characteristics of target microRNAs, such as tissue-specificity, should be considered and the sampling site for microRNAs should be presented in forensic applications."

Journal • Cardiovascular

Plasma exosome-derived microRNAs expression profiling and bioinformatics analysis under cross-talk between increased low-density lipoprotein cholesterol level and ATP-sensitive potassium channels variant rs1799858. (PubMed, J Transl Med) - "These results firstly indicated the plasma exo-miRs expression profile bridging the link between genotype (KATP rs1799858) and phenotype (higher LDL-C serum level), these 5 DE-exo-miRs may be potential target intermediates for molecular intervention points."

Journal • Dyslipidemia • MIR126 • MIR200 • MIR200A • MIR210 • MIR221 • MIR320A • MIR4664 • MIR490

Production of Cardiomyocytes by microRNA-Mediated Reprogramming in Optimized Reprogramming Media. (PubMed, Methods Mol Biol) - "In contrast, we utilize a combination of four miRNAs (miR-1, miR-133, miR-208, miR-499) that we call miR Combo. One common issue regarding direct cardiac reprogramming strategies is low efficiency. Recently, we have demonstrated that the efficiency of direct cardiac reprogramming is enhanced in the chemically defined reprogramming media."

Journal

99mTc-MIRC208 SPECT/CT Imaging for HER2-Positive Cancer Patients (clinicaltrials.gov) - P=N/A; N=200; Recruiting; Sponsor: Beijing Cancer Hospital

Clinical • New trial • Oncology • HER-2

Carvedilol Alters Circulating MiR-1 and MiR-214 in Heart Failure. (PubMed, Pharmgenomics Pers Med) - "Here we proposed that the expression of hypertrophic-specific miRNAs (miR-1, miR-133, miR-208, and miR-214) might be linked to beneficial effects of carvedilol. MiR-1 was significantly downregulated in the untreated group compared with healthy individuals (P=0.019 and 0.14-fold). In conclusion, it might be postulated that one of the mechanisms by which carvedilol may exert its cardioprotective effects can be through increasing miR-1 and miR-214 expressions which may also serve as a potential therapeutic target in patients with systolic HF in future."

Journal • Cardiovascular • Congestive Heart Failure • Fibrosis • Heart Failure • Immunology

[VIRTUAL] Early overexpression of miR-499 in non-ST elevation acute coronary syndromes predicts long-term risk of major adverse cardiac events (ESC 2020) - "Aim: To evaluate the prognostic value of five circulating miRs (miR-1, miR-21, miR-133a, miR-208, miR-499) levels for predicting major adverse cardiac events (MACE), including death, nonfatal myocardial infarction (MI) or cardiovascular rehospitalization (reh.) in patients with NSTE-ACS... Elevated miR-499 levels independently of high sensitivity troponin T levels in early phase of NSTE-ACS are related to increased rate of MACE in 4-year follow-up."

Adverse events • Acute Coronary Syndrome • Cardiovascular • Coronary Artery Disease • Myocardial Infarction • MIR21

miR-1, miR-499 and miR-208 are sensitive markers to diagnose sudden death due to early acute myocardial infarction. (PubMed, J Cell Mol Med) - "Real-time PCR results showed a down-regulation of all miRNAs in the AMI group compared with SCD and control. The selected miRNAs presented high accuracy in discriminating SCD from AMI (miR-1 and miR-499) and AMI from control (miR-208) representing a potential aid for both clinicians and pathologists for differential diagnosis."

Biomarker • Journal • Cardiovascular • Myocardial Infarction

Role of microRNAs in myogenesis and their effects on meat quality in pig-Review. (PubMed, Asian-Australas J Anim Sci) - "Various kinds of microRNAs (miRNAs), miR-1, miR-21, miR22, miR-27, miR-34, miR-127, miR-133, miR-143, miR-155, miR-199, miR-206, miR-208, miR-378, and miR-432 play important roles in pig skeletal muscle development. Further, the quality of meat also depends upon myofiber which is developed through the expression of different kinds of miRNAs at different stages. This review will focus on the mechanism of myogenesis, the role of miRNAs in myogenesis, and meat quality with a focus on the pig."

Journal • MIR155 • MIR21

miR-208b-5p inhibits invasion of non-small cell lung cancer through the STAT3 pathway by targeting interleukin-9. (PubMed, Oncol Lett) - "Previous studies reported a dysregulation of micro (mi)R-208b-5p expression level in various types of human cancer; however, the role of miR-208-5p in non-small cell lung cancer (NSCLC) remains unclear...In conclusion, the findings from this study demonstrated that miR-208b-5p inhibited migration and invasion of NSCLC cells. The anti-tumor activity of miR-208b-5p may be mediated by IL-9 and STAT-3 pathway."

Journal • Lung Cancer • Non Small Cell Lung Cancer • Oncology • Solid Tumor • Thoracic Cancer • IL9 • PCR

The Functions of microRNA-208 in the Heart. (PubMed, Diabetes Res Clin Pract) - "However, the functions and underlying mechanisms of miR-208 in heart are still unclear. In this review, we highlighted the novel insights of miR-208 functions and associated mechanisms in the regulation of cardiac diseases."

Journal • Review

Disease severity-related alterations of cardiac microRNAs in experimental pulmonary hypertension. (PubMed, J Cell Mol Med) - "We hypothesized that heart-relevant microRNAs, that is myomiRs (miR-1, miR-133a, miR-208, miR-499) and miR-214, can have a role in the right ventricle in the development of PAH...The myomiRs negatively correlated with Fulton index as a measure of RV hypertrophy in MCT group (P < 0.05), whereas miR-214 showed a positive correlation (P < 0.05). We conclude that the expression of determined microRNAs mirrored the disease severity and targeting their pathways might represent potential future therapeutic approach in PAH."

Journal • Cardiovascular • Hypertension • Pulmonary Arterial Hypertension • Pulmonary Disease • Respiratory Diseases

The whole-transcriptome landscape of muscle and adipose tissues reveals the ceRNA regulation network related to intramuscular fat deposition in yak. (PubMed, BMC Genomics) - "Taken together, our results reveal the potential mRNA and noncoding RNAs involved in IMF deposition in the yak, providing a useful resource for further research on IMF deposition in this animal species."

Journal

Circulating miR-320a as a Predictive Biomarker for Left Ventricular Remodelling in STEMI Patients Undergoing Primary Percutaneous Coronary Intervention. (PubMed, J Clin Med) - "Moreover, the expression of miR-29a, miR-29b, miR-324, miR-208, miR-423, miR-522, and miR-545 was differentially expressed even before PPCI in STEMI. Furthermore, the increase in circulating miR-320a and the decrease in its expression in PBMCs were significantly associated with LVAR and correlated with the expression of miR-320a in human failing myocardium from ischaemic origin. In conclusion, we determined the time course expression of new circulating miRNAs in patients with STEMI treated with PPCI and we showed that miR-320a was positively associated with LVAR."

Biomarker • Clinical • Journal

MicroRNA-331 and microRNA-151-3p as biomarkers in patients with ST-segment elevation myocardial infarction. (PubMed, Sci Rep) - "Importantly, this was observed in patients presenting early with STEMI, even before the markers of myocardial necrosis (cardiac troponin I, miR-208 and miR-499) were elevated, which suggests that the origin of miR-331 and miR-151-3p might be in the VP. In conclusion, the study provides two novel biomarkers observed in STEMI, which may be associated with plaque rupture."

Biomarker • Clinical • Journal • TNNI3

Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis. (PubMed, Sci Rep) - "Although NSTEMI modified the expression of miR-1 and miR-208 in both serum and plasma, plasma displayed a higher variance than serum (Levene's test p < 0.01)...Interestingly, miR-21 showed an opposite pattern of expression, being increased in serum (2: 5.7, p = 0.0221) and decreased in plasma (2: 0.5, p = 0.0107). Plasma and serum exhibit different patterns of circulating miRNA expression in NSTEMI and suggest that results from studies with different starting material could not be comparable."

Clinical • Journal • MIR21