K201 / Sequel - LARVOL DELTA

Single Centre Experience of Transbronchial Peripheral Lung Nodule Biopsy with Bronchial Branch Tracing, Radial Miniprobe and Fluoroscopy (APSR 2025) - "All patients underwent moderate sedation with midazolam, fentanyl or pethidine. Bronchoscopies were performed with Olympus 1TH1100 with K203 guide sheath and 20R radial ultrasound, or P190 with K201 and 17S radial ultrasound, or MP 190 with 17s radial ultrasound...The solitary patient without specific diagnosis is currently on follow up with repeat CT imaging. Conclusion : Bronchial branch tracing, radial endobronchial ultrasound and fluoroscopy remains a feasible technique with good diagnostic yield for evaluation of peripheral lung nodules."

Biopsy • Clinical • Anesthesia

Granzyme B PET Imaging Enhances Belzutifan and KL201 Combination Therapy Using a Tumor Cell Membrane Nano-Loading Platform for Renal Cell Carcinoma (EANM 2025) - "The nanoplatforms demonstrated uniform morphology, optimal size, and excellent stability. In vivo, the combination treatment significantly inhibited tumor growth compared to monotherapies, underscoring the potential of this nano-loaded platform to improve RCC treatment outcomes."

Combination therapy • Tumor cell • Genito-urinary Cancer • Oncology • Renal Cell Carcinoma • Solid Tumor • CRY1 • EPAS1 • GZMB

Pharmacological study of the safety, efficacy, and potential of M201-A on paroxysmal and persistent atrial fibrillation. (PubMed, J Pharmacol Exp Ther) - "It was the most active conversion metabolite when K201 was administered orally in patients with paroxysmal AF. These pharmacological effects of M201-A, combined with its inhibitory actions on RyR2, provide a promising therapeutic option for treating AF."

Journal • Atrial Fibrillation • Cardiovascular

Establishment of a Visible Reporter System in Zymomonas mobilis through Random Mutagenesis and Rational Design of a Chromoprotein eforRed. (PubMed, ACS Synth Biol) - "Mechanistic studies revealed that substitutions at the amino acid residues K201 and T24 situated on the surface region of eforRed, especially the double-mutant K201H-T24V, could optimize the protein optical characteristics by concurrently increasing intrinsic fluorescence and elevating protein expression levels. Finally, a visible reporter-gene system was successfully established in Z. mobilis by expressing the mutant eforRedK201H-T24V under a strong promoter like Pgap-4S or PT7 that gave colonies a visible red color for phenotypic screening and retained high fluorescence intensity for quantitative analysis. This study thus not only constructed a robust visible reporter-gene system in the non-model bacterium Z. mobilis but also helped expand the applications of chromoproteins in biotechnology and synthetic biology, especially in non-model microorganisms."

Journal • Infectious Disease

Granzyme B PET Imaging Enhances Belzutifan and KL201 Combination Therapy Using a Tumor Cell Membrane Nano-Loading Platform for Renal Cell Carcinoma (SNMMI 2025) - "This study successfully developed a tumor cell membrane-based nanoplatform co-delivering Belzutifan and KL201 for the treatment of RCC. The combination therapy enhanced the tumor immunocidal effect by boosting the immune response at the tumor site. The nanoplatforms demonstrated uniform morphology, optimal size, and excellent stability."

Combination therapy • Tumor cell • Genito-urinary Cancer • Oncology • Renal Cell Carcinoma • Rhabdomyosarcoma • Sarcoma • Solid Tumor • CRY1 • EPAS1 • GZMB

Simultaneous optical recording of action potentials and calcium transients in cardiac single cells differentiated from type 1 CPVT-iPS cells. (PubMed, Front Physiol) - "In drug-loading experiments on CPVT1 (RyR2-I4587V) hiPSC-derived ventricular-like CMs, carvedilol and flecainide demonstrated some effectiveness, while JTV519 at 3 µM exhibited both efficacy and alterations in AP waveforms. The Ca2+/calmodulin-dependent serine-threonine protein kinase II (CaMKII) inhibitor KN-93 at 1 µM was highly effective (93%) at reducing Ca2+ transient abnormalities without altering AP waveforms."

Journal • Cardiovascular • Ventricular Tachycardia • CYP19A1 • PVT1

Dysregulation of CRY1 impairs brain thyroid hormone pathway and promotes anxiety-like behavior in male mice. (PubMed, Metabolism) - "These findings underscore the critical role of CRY1 in regulating thyroid hormone and anxiety, providing insight into the underlying pathogenesis and potential treatment strategies for mood disorders."

Journal • Preclinical • CNS Disorders • Mood Disorders • Psychiatry • CLOCK • CRY1 • SLCO1C1

Development of novel InDel markers by whole-genome sequence comparison and genetic diversity assessment of Thailand rice blast fungus populations. (PubMed, Stress Biol) - "The effectiveness of these 82 loci was assessed by analyzing the genetic diversity of 47 Thai rice blast isolates alongside two reference isolates, GUY11 (France) and KJ201 (Korea)...This finding suggests a correlation between genetic relatedness and the plant hosts susceptible to rice blast disease. The newly developed InDel markers provide a valuable resource for future research in this field."

Journal

c-di-GMP inhibits rRNA methylation and impairs ribosome assembly in the presence of kanamycin. (PubMed, EMBO Rep) - "Molecular dynamics simulation and mutagenesis studies reveal that c-di-GMP binds to RlmI at residues R64, R103, G114, and K201...Binding and methylation assays indicate that the inhibitory effect of c-di-GMP on RlmI is conserved across various pathogenic bacteria. Our data suggest an unexpected role for c-di-GMP in regulating ribosome assembly under stress through the inhibition of rRNA methyltransferases."

Journal

Disruption of Ca2+ Homeostasis and Enhanced Ca2+ Signaling in Scleroderma Fibroblasts Is Due to the Vicious Cycle Formed by Mitochondrial-derived Reactive Oxygen Species, CaMKII, and ER Extrusion Pump RYR2 (ACR Convergence 2024) - "Fibroblasts were treated with a CaMKII inhibitor KN93, a mitoROS quencher Mitoquinol (MitoQ), or a RYR2 stabilizer JTV519 for 72 hours. Our research revealed a new paradigm where intracellular Ca2+ and CaMKII foster a pro-fibrotic profile in SSc fibroblasts. We provided evidence that the dual activation of CaMKII – via Ca2+/calmodulin binding and oxidative modulation – amplifies Ca2+ signaling, which in turn drives the fibrotic phenotype. These insights significantly extend the current understanding of Ca2+-related mechanisms in SSc fibrosis, a previously underexplored area."

Fibrosis • Immunology • Scleroderma • Systemic Sclerosis • CAMK2A

Mitochondrial MOF regulates energy metabolism in heart failure via ATP5B hyperacetylation. (PubMed, Cell Rep) - "We demonstrate that the acetylation of ATP5B at K201, co-regulated by MOF and SIRT3, impairs mitochondrial respiration and energy metabolism both in vitro and in vivo. These findings suggest that the role of MOF in HF could be attributed to its regulation of ATP5B acetylation. Overall, our results highlight the disruptive impact of mitochondrial MOF on cardiac function and emphasize the significance of enzyme-catalyzed acetylation in mitochondria."

Journal • Cardiovascular • Congestive Heart Failure • Heart Failure • SIRT3

In vivo cardiovascular profile of ryanodine receptor 2 inhibitor M201-A: Utility as an anti-atrial fibrillatory drug for patients suffering from heart failure with preserved ejection fraction. (PubMed, J Pharmacol Sci) - "M201-A prolonged J-Tpeakc without prolonging Tpeak-Tend or terminal repolarization period, indicating the risk of causing torsade de pointes is negligible. Thus, M201-A is expected to become a hopeful therapeutic strategy for patients having pathology of both AF and HFpEF."

Journal • Preclinical • Anesthesia • Atrial Fibrillation • Cardiovascular • Congestive Heart Failure • Heart Failure

Mutations in fibulin-1 and collagen IV suppress the short healthspan of mig-17/ADAMTS mutants in Caenorhabditis elegans. (PubMed, PLoS One) - "Interestingly, fbl-1(k206), but not fbl-1(k201) or let-2 alleles, exhibited an extended lifespan compared to the wild type when combined with mig-17. These results reveal allele specific interactions between let-2 or fbl-1 and mig-17 in age-related phenotypes, indicating that basement membrane physiology plays an important role in organismal aging."

Journal • FBLN1

Polyaminated, acetylated and stop codon readthrough of recombinant Francisella tularensis universal stress protein in Escherichia coli. (PubMed, PLoS One) - "Of the 24 lysines in rUsp/His6, 10 were acetylated (K63, K68, K72, K129, K175, K201, K208, K212, K233, and K238) and three of the four glutamines had putrescine, spermidine and spermine adducts (Q55, Q60 and Q267)...LC-MS/MS revealed that stop codon readthrough had occurred resulting in the unexpected addition of 20 extra amino acids at the C-terminus of rUsp/His6, after the histidine tag. Further, the finding of polyaminated glutamines in rUsp/His6 indicated that E. coli is capable of transglutaminase activity."

Journal

Introgression of OsAP47 by marker-assisted selection enhanced resistance against southern rice black-streaked dwarf virus disease. (PubMed, Virology) - "The results indicated that introgression of OsAP47 improved rice resistance and can avoid yield losses produced by SRBSDVD. KH201 was demonstrated as a resistance material that could be used in rice breeding."

Journal • Infectious Disease

Cryptochrome 1 regulates ovarian granulosa cell senescence through NCOA4-mediated ferritinophagy. (PubMed, Free Radic Biol Med) - "KL201, a Cry1 stabilizer, enhanced ovarian function in naturally aged mice by reducing ferritinophagy. Our study reveals the potential mechanisms of action of Cry1 during ovarian aging and provides new insights for the clinical treatment of age-related fertility decline."

Journal • Infertility • Sexual Disorders • Targeted Protein Degradation • CRY1 • NCOA4

Host-induced gene silencing is a promising biological tool to characterize the pathogenicity of Magnaporthe oryzae and control fungal disease in rice. (PubMed, Plant Cell Environ) - "In this study, the HIGS system of M. oryzae was established using transgenic fungus expressing green fluorescence protein (GFP), KJ201::eGFP and 35S::dsRNAi plants, which produce small interfering RNAs targeting fungal genes...Overall, the results of this study demonstrate that HIGS is a very effective and efficient biological tool not only to accurately characterize the functions of fungal pathogenic genes during rice-M. oryzae interactions, but also to control fungal disease and ensure a successful rice production."

Journal • Infectious Disease

iTRAQ-based protein profiling and functional identification of four genes involved in rice basal resistance against Magnaporthe oryzae in two contrasting rice genotypes. (PubMed, Stress Biol) - "Here, from the comparisons of differentially expressed proteins (DEPs) of NPB-Piz-t to the avirulent isolate KJ201 (KJ201-Piz-t)and the virulent isolate RB22 (RB22-Piz-t) with mock-treated NPB-Piz-t (Mock-Piz-t), NPB to the virulent isolate KJ201(KJ201-NPB) and RB22 (RB22-NPB) with mock-treated NPB (Mock-NPB), 1, 1, and 6 common DEPs were, respectively, identified at 24, 48 and 72 h post-inoculation (hpi) in the susceptible comparisons of RB22-Pizt/Mock-Piz-t, KJ201-NPB/Mock-NPB, and RB22-NPB/Mock-NPB, involving in gi|54,290,836 and gi|59,800,021 were identified in the resistance comparison KJ201-Piz-t/Mock-Piz-t at 48 and 72 hpi respectively...Further, expression and targeted metabolomics analysis illuminated the resistance response of cysteine-containing substances as gi|59,800,021 under blast infection. These results provide new targets for basal resistance gene identification and open avenues for developing novel rice blast resistant materials."

Journal • Infectious Disease

Preoperative Localization Technique Using Mixture of Indigo-Carmine and Lipiodol of Pulmonary Nodule via Transbronchial Approach (IASLC-ACLC 2022) - "Bronchoscopy was performed using a 4.0-mm flexible bronchoscope (BF-P260F; Olympus,Tokyo, Japan )under conscious sedation using fentanyl and midazolam...Thereafter, under X-ray fluoroscopic guidance, a 20-MHz radial probe EBUS (UM-S20-17S; Olympus, Tokyo, Japan) was inserted into theguide sheath (K-201; Olympus, Tokyo, Japan) and pushed through the working channel towards and as close to the lesion as possible (Figure 1)...2), and 2/60 required c-arm assistance. None of the patients complained of discomfort during the procedure, and in all cases, margins were found to be free from carcinoma as determined by final pathology Results .ConclusionWe recommend this localization technique using mixture of indigo-carmine and lipiodol in concert with the transbronchial approach because the procedure time is short, patient's discomfort is low, and success rate is high."

Anesthesia • Oncology

Improved Antioxidative, Anti-Inflammatory, and Antimelanogenic Effects of Fermented Hydroponic Ginseng with Bacillus Strains. (PubMed, Antioxidants (Basel)) - "This study aimed to enhance the multifunctional effects, including the antioxidative, anti-inflammatory, and antimelanogenic effects, exhibited by fermented HG with Bacillus strains, such as Bacillus subtilis KU43, Bacillus subtilis KU201, Bacillus polyfermenticus SCD, and Bacillus polyfermenticus KU3, at 37 °C for 48 h. After fermentation by B. subtilis KU201, the antioxidant activity, determined using ABTS and FRAP assays, increased from 25.30% to 51.34% and from 132.10% to 236.27%, respectively, accompanied by the enhancement of the phenolic compounds and flavonoids...These results indicate that the HG exhibited stronger antioxidative, anti-inflammatory, and antimelanogenic effects after fermentation. Consequently, HG fermented by Bacillus strains can potentially be used as an ingredient in cosmetological and pharmaceutical applications."

Journal • Immunology • Inflammation • Oncology • IL6 • TNFA • Tyrosinase

Tooth color in dental students from Buenos Aires University, Dental School, Argentina. (PubMed, Acta Odontol Latinoam) - "A dental prophylaxis procedure was performed on the buccal surface of each 1.1 tooth with a prophylaxis brush (TDV) mounted on a low-speed rotary instrument Kavo 2068 CHC (Germany) micromotor and a Kavo LUX K201(Germany) contra-angle...Shades D1 and C1 were not determined in any subject. Within the conditions of this study, A1 was the most prevalent shade in central incisors, followed by A2 and B2."

Journal • Musculoskeletal Diseases • Orthopedics

Analysis of a rice blast resistance gene Pita-Fuhui2663 and development of selection marker. (PubMed, Sci Rep) - "Here, we identified a resistance gene from the rice cultivar Fuhui 2663 which is resistant to the rice blast isolate KJ201...Importantly, Pita-Fuhui2663 did not affect the main agronomic traits of the variety compared to the Pita gene as verified by knockout experiments, indicative of potential applications of Pita-Fuhui2663 in broader breeding programs. Furthermore, a Pita-Fuhui2663-dCAPS molecular marker with good specificity and high efficiency was developed to facilitate rice breeding for resistance to this devastating disease."

Journal • Psychiatry

JTV-519 Alleviates the Impairment of FGF21 Axis Signaling after Spinal Cord Contusion in Mice (ENDO 2022) - "These data imply that JTV-519 is able to alleviate SCI-induced impairments of FGF21/ adiponectin signaling. Therefore, JTV519 could be a potential pharmacological agent to treat metabolic dysfunction after SCI."

Preclinical • CNS Disorders • Metabolic Disorders • Orthopedics • FGF21

Hypoxia-Induced Sarcoplasmic Reticulum Ca2+ Leak Is Reversed by Ryanodine Receptor Stabilizer JTV-519 in HL-1 Cardiomyocytes. (PubMed, Anatol J Cardiol) - "Hypoxia increased sarcoplasmic reticulum calcium leakage which was ame- liorated by JTV-519 treatment independently of gene or protein expression. JTV-519 rep- resents a possible treatment for obstructive sleep apnea-associated HF."

Journal • Cardiovascular • Congestive Heart Failure • Heart Failure • Obstructive Sleep Apnea • Respiratory Diseases • Sleep Disorder

Field evaluation of the enhanced MM3-COPRO ELISA test for the diagnosis of Fasciola hepatica infection in sheep. (PubMed, PLoS One) - "The enhanced MM3-COPRO test (eMM3-COPRO) and the commercial version BIO K 201 (Bio-X Diagnostics, Rochefort, Belgium) are widely used as immunodiagnostic tools for the specific detection of coproantigens released by Fasciola during the late prepatent and patent stages of infection...The test also proved highly specific as coproantigens disappeared after the treatment. The eMM3-COPRO was less time consuming than coproscopy, particularly when the procedure involved numerous samples, and showed promise as a tool for monitoring flukicide efficacy."

Journal • Hepatology • Infectious Disease