Stealth Vector HGTV43 (anti-HIV-1 antisense RNA) / Enzo - LARVOL DELTA

High resolution structural characterization of a new AAV5 antibody epitope toward engineering antibody resistant recombinant gene delivery vectors. (PubMed, J Virol) - "This identified variants with antibody escape phenotypes that maintain infectivity.IMPORTANCE Biologics based on recombinant AAVs (rAAVs) are increasingly becoming attractive human gene delivery vehicles, especially after the approval of Glybera in Europe and Luxturna in the United States. The resolution of the density map enabled the identification of interacting residues between capsid and antibody, and the determinants of neutralization. The information obtained from this study can thus facilitate the generation of host immune escape vectors."

Journal

Human Polyclonal Anti-AAV Neutralizing Antibody Epitope Mapping by NGS Identifies Common Epitopes and Enables the Design of Stealth Mutants (ASGCT 2019) - "In summary, we demonstrate that IP-Seq combined with PK-Seq is an effective non-traditional method to identify conformational epitopes of polyclonal anti-AAV NAbs and allows the rational design of stealth vectors. This method involves only standard molecular biology techniques and can be adopted by a broad range of labs and utilized in theory for any serotypes and variants besides AAV2."

Next-Generation Sequencing