chlorogenic acid / Jiuzhang Biotech - LARVOL DELTA

Metabolomic Analysis of Tomato Plants Treated With Garlic Extract, Bacillus subtilis, and Their Combination for Defense Against Bacterial Wilt. (PubMed, Plant Environ Interact) - "The profiling revealed that garlic extract up-regulated key phenolic compounds, including chlorogenic acid and caffeoyl glucaric acid, which contribute to pathogen defense by reinforcing cell structures and mitigating oxidative stress...These findings indicate that garlic extract enhances plant defense primarily through phenylpropanoid-mediated structural reinforcement, while B. subtilis contributes to disease suppression through microbial interactions rather than significant metabolic shifts. Understanding these metabolic trade-offs offers valuable insights into optimizing bacterial wilt management strategies, ultimately improving tomato resilience and productivity."

Journal • Infectious Disease

Focusing on the impact of chlorogenic acid covalent binding on the fibrillation properties of pea vicilin and legumin globulins. (PubMed, Int J Biol Macromol) - "Moreover, covalently bound CGA endowed both protein fibers with enhanced antioxidant capacity in a concentration-dependent manner. Overall, these results demonstrate that covalent polyphenol modification can effectively regulate the fibrillation properties of pea 7S and 11S globulins, thereby expanding the functionality of pea protein derived products."

Journal

Bioassay-guided identification of Chlorogenic Acid as an anti-snake venom constituent of Ficus racemosa L.: An in vitro and in silico study. (PubMed, J Ethnopharmacol) - "This is the first mechanistic in vitro characterization of the anti-ophidic properties of F. racemosa and identification of CGA as the active constituent. These findings require further in vivo validation, pharmacokinetic evaluation, safety assessment, and synergistic formulation to translate mechanistic insights into clinically viable therapeutic interventions for management of snakebite envenomation."

Journal • Preclinical

Simultaneous Recovery of Mannooligosaccharides and Chlorogenic Acids from Spent Coffee Grounds: Integration of Enzymatic Hydrolysis with Natural Deep Eutectic Solvent Extraction. (PubMed, J Agric Food Chem) - "Extraction with choline chloride-citric acid NADES achieved the highest MOS and CGA yields at 114.81 and 2.11 mg/g of SCG, respectively, with an antioxidant capacity of 0.14 mmol of Trolox equivalent/g of SCG. This work demonstrates that integrating biocatalysis with solvent informatics can overcome matrix and polarity limitations, offering a sustainable strategy for upcycling food waste into functional ingredients without generating harmful residues."

Journal

A comparative analysis of bioactive phytochemicals in Cynanchum viminale (L.) L. and Pergularia daemia (Forssk.) Chiov. from Saudi Arabian folk medicine. (PubMed, Naturwissenschaften) - "Molecular docking brilliantly illuminated the mechanism behind this divergence: the potent cytotoxicity of C. viminale is driven by its principal alkaloids, Protopine and Berberine, which showed high-affinity binding to the ATP-binding site of EGFR, a key driver of cancer proliferation. DHFR, conversely, was identified as a common molecular target for potent binders from both plants (Evodiamine, Piperine, Chlorogenic acid), providing a unifying mechanism for their shared antimicrobial properties."

Clinical • Journal • Liver Cancer • Oncology • Solid Tumor • DHFR • EGFR

Assessment of the Antimicrobial Activity of Cistus salviifolius L. and Helichrysum stoechas (L.) DC Extracts and Their Synergistic Potential with Conventional Antibiotics Against Staphylococcus aureus. (PubMed, Int J Mol Sci) - "When combined with antibiotics such as ampicillin, ciprofloxacin, or vancomycin, the extracts of C. salviifolius and H. stoechas predominantly exhibited synergistic (FICI value ≤ 0.5) or additive effects (0.5 < FICI ≤ 1), with some combinations resensitizing resistant strains...The presence of compounds such as myricetin 3 O-galactoside, catechin derivatives, gallic acid, kaempferol, and chlorogenic acid in the extracts may contribute to their anti-Staphylococcus activity. These results demonstrated the dual antimicrobial and antivirulence potential of C. salviifolius and H. stoechas extracts, highlighting their promise as therapeutic agents or adjuvants against S. aureus. These extracts can be promising candidates for further studies on the development of novel strategies targeting multiple pathogenic pathways."

Journal

Ethanolic Extract of Stachys byzantina Leaf: Optimization of Ultrasonic Probe-Assisted Extraction and Characterization. (PubMed, Plants (Basel)) - "Nicotinic acid was also detected in the extracts, with levels ranging from 11.91 to 13.86 mg/100 g. The fatty acid profile indicated the presence of lauric, palmitic and linolenic acids in higher concentrations, with quantification of squalene, α-tocopherol and β-sitosterol. The ethanolic extract of Stachys byzantina showed no cytotoxic effect on HaCaT cells at concentrations up to 200 µg/mL, maintaining cell viability above 70% after 48 h of exposure."

Journal

Bioaccessibility and Gut Microbiota Modulation of Phenolics in Prunus mume vs. Fructus mume. (PubMed, Foods) - "Twenty-four individual phenolics were found after gastrointestinal digestions with neochlorogenic acid and chlorogenic acid as the predominant ones...Notably, FM has a better effect on regulating the gut microbiota composition than PM, as evidenced by a greater enrichment of beneficial bacteria such as Bifidobacterium and Megamonas, along with a stronger suppression of the pathogenic Escherichia-Shigella. These results provided insights into the digestive properties of polyphenols from PM and FM, indicating that processing of PM into FM potentially enhance its health-improving effects on the colon."

Journal

The Double Life of Plant-Based Food Waste: A Source of Phenolic Acids and a Carrier for Immobilization of Lipases Capable of Their Lipophilization. (PubMed, Int J Mol Sci) - "Chlorogenic acid (CGA), predominant in extracts, served as a model substrate...While lipophilization of chokeberry pomace and spent coffee grounds extracts reduced antioxidant activity, short-chain esters (C4-C6) improved rapeseed oil stability. The findings highlight food waste as a sustainable source for developing biocatalysts and value-added bioactives with enhanced functional properties."

Journal • Infectious Disease

Integrated Transcriptomic and Metabolomic Analysis Reveals Biochar-Induced Enhancement of Growth and Secondary Metabolism in the Medicinal Plant Echinacea purpurea. (PubMed, Int J Mol Sci) - "Metabolomic profiling further revealed a pronounced upregulation of phenylpropanoid pathway metabolites, including 3-hydroxy-4-methoxycinnamic acid, chlorogenic acid methyl ester, ferulic acid O-hexoside, and coumarin derivatives such as 7-methoxy-4-methylcoumarin...These integrated results reveal the mechanistic basis through which biochar application simultaneously promotes growth and enhances the secondary metabolism of E. purpurea by coordinately activating phytohormone signaling and phenylpropanoid biosynthesis. These results establish the potential of biochar in enhancing E. purpurea cultivation, with future work needed to determine optimal application rates."

Journal

Synergistic Effects of Selenium and Silicon Nanoparticles on Peach Quality Enhancement and Se Biofortification Through Foliar Application. (PubMed, Plants (Basel)) - "Se5Si10 led to the highest antioxidant enzyme activities (peroxidase: 2254 U g-1, catalase: 61.7 U g-1) and phenolic compound enhancement (chlorogenic acid: 267 mg kg-1, total phenolics: 12.8 mg GAE g-1)...Combined treatments reduced oxidative stress markers (MDA: 22.11 μmol g-1) while enhancing protein metabolism. These findings demonstrate that Se-Si nanoparticle combinations showed optimal biofortification with synergistic quality enhancement, establishing effective strategies for nutritionally enriched peach production."

Journal • CAT

Impact of greenhouse vs. open field cultivation on volatiles, phenolics, organic acids, antimicrobial activity, phenology, and molecular bioactivity of kumquat (Fortunella margarita) fruits. (PubMed, Food Chem) - "Molecular docking indicated strong chlorogenic acid and quercetin binding affinities to key microbial enzymes (-130.46 and -100.17 kcal⸳mol-1, respectively). Toxicity predictions indicated low toxicity for most compounds. These findings underscore the key role of cultivation environment in modulating functional and biochemical attributes of kumquat."

Journal

Targeting JAK2/STAT3-Dependent Macrophage Polarization by Chlorogenic Acid Attenuates Hepatic Inflammation in Chronic Stress. (PubMed, Cells) - "Chronic stress adversely affects and compromises physiological well-being in humans, inducing hepatic injury, with its pathogenesis mechanistically linked to alterations in macrophage polarization and the regulation of the inflammatory microenvironment. Additionally, the use of the JAK2/STAT3 signaling pathway inhibitor, S3I-201, demonstrated effects similar to those observed with CGA treatment. In summary, CGA modulates macrophage polarization and the inflammatory response through the regulation of the JAK2/STAT3 signaling pathway, thereby mitigating the liver injury induced by chronic stress."

Journal • Hepatology • Inflammation • Liver Failure • IL10 • IL1B • IL6 • JAK2 • STAT3 • TNFA

Comparative analysis of the biochemical composition of fruit tissues in representatives of the Malus sect. Chloromeles. (PubMed, Protoplasma) - "Specifically, our research revealed the absence of procyanidin B1 and a high content of chlorogenic acid, accompanied by remarkably low quantities of cinnamic acid and its derivatives...The observed composition, including elevated transport sugar and protopectin levels, is typically associated with fruits at early ripening stages. This may contribute to the suboptimal flavor development, despite the presence of a relatively high total soluble sugar content."

Journal

Investigating a new Dillenia ovata endophytic bacteria to produce antioxidants and anti-diabetes activity in vitro and in vivo. (PubMed, Biotechnol Rep (Amst)) - "DO-R5 significantly enhanced the production of bioactive compounds including diosmin, rutin, and chlorogenic acid, and exhibited improved in vitro antioxidant, anti-inflammatory, and anti-diabetic activities. In vivo, the extract effectively reduced blood glucose, regulated lipid profiles, supported weight control, and protected organs. These findings emphasize the potential therapeutic use of D. ovata endophytic bacteria as a natural source of anti-diabetic drugs."

Journal • Preclinical • Diabetes • Metabolic Disorders

From phyllosphere to fermentation: Impact of fermentation scale and temperature on sauerkraut fermentation. (PubMed, Int J Food Microbiol) - "The organic acid concentrations and the metabolism of phenolic compounds differed depending on whether Lactiplantibacillus, Leuconostoc or Rahnella were the predominant fermentation microorganisms. Collectively, our data highlight that the establishment of LAB communities in sauerkraut fermentation is influenced by both the fermentation scale and temperature, underscoring the critical roles of Lactiplantibacillus at higher temperatures and Leuconostoc at lower temperatures."

Journal

Inhibitory effect of Vaccinium arctostaphylos L. and Vaccinium myrtillus L. extracts on melanogenesis in α-MSH-induced B16F10 melanoma cells: in vitro, molecular docking ADMET and drug-likeness studies. (PubMed, J Sci Food Agric) - "The fruit extracts of V. arctostaphylos in particular appear to be a potential and promising source for the cosmeceutical and pharmaceutical industry due to their content of active ingredients and strong biological effect."

Journal • Preclinical • Dermatology • Melanoma • Oncology • Solid Tumor • Tyrosinase

Zinc-chlorogenic acid network-induced tomato seed protein gels: Fabrication, characterization, and application in a plant-based food. (PubMed, Food Res Int) - "In vitro digestion revealed superior digestibility for PBC, with free amino acid content increasing 2.83-fold post-digestion. This work establishes ZCN as an effective strategy for reinforcing TSP gels and provides a sustainable approach to valorize tomato seed waste for high-quality plant-based dairy alternatives."

Journal

Food-derived chlorogenic acid prevents aortic aneurysm and dissection by nutritional restore branched-chain amino acid dyshomeostasis. (PubMed, J Nutr Biochem) - "Crucially, CGA directly targeted and inhibited LAT3, thereby normalizing BCAA metabolism and suppressing mTOR activity, which mitigated AAD progression. Our findings not only reveal the therapeutic potential of CGA in AAD prevention but also identify the LAT3/BCAA/mTOR axis as a novel mechanistic target for AAD intervention."

Journal • Cardiovascular

Protective Effects of Standardized Peucedanum Japonicum Thunb. Extract Against UVB-Induced Corneal Epithelial Apoptosis via Mitochondrial Regulation. (PubMed, Front Biosci (Landmark Ed)) - "SBP protects corneal cells from UVB-induced apoptosis through mitochondrial stabilization, suggesting its potential as a therapeutic agent for ocular surface disorders."

Journal • Corneal Abrasion • Metabolic Disorders • Ophthalmology

Therapeutic and Safety Evaluation of Enhydra fluctuans Lour. and Ipomoea aquatica Forssk. as Acetylcholinesterase, Butyrylcholinesterase, β-Secretase, and CytochromeP450 Inhibitors Using In Vitro and UPLC-QTOF-MS-Based Molecular Docking and Dynamic Simulation Study. (PubMed, Chem Biodivers) - "The UPLC-QTOF-MS analysis tentatively identified phytometabolites from the phenylethanoid glycosides and chlorogenic acids class in both the extracts. Further, in silico toxicity prediction, molecular docking, and dynamic simulation studies provided additional evidence on the safety profile and interaction potential of phytometabolites with AChE, BChE, and BACE1 enzymes."

Journal • Preclinical • Alzheimer's Disease • CNS Disorders • Hepatocellular Cancer • Oncology • Pain • Solid Tumor

Chlorogenic acid-rich Morus alba leaf alleviated renal fibrosis through the regulation of transforming growth factor-beta 1 (TGF-β1) and fibroblast growth factor-2 (FGF-2), and extracellular matrix deposition. (PubMed, Mol Biol Rep) - "These findings suggest that MLE and CGA effectively alleviate renal fibrosis by modulating crucial profibrotic signaling pathways, presenting them as promising natural therapeutic alternatives for CKD-associated fibrosis."

Journal • Chronic Kidney Disease • Fibrosis • Immunology • Nephrology • Renal Disease • CTNNB1 • FGF2 • FGFR2 • SMAD3 • TGFB1 • TGFBR1

Chlorogenic acid alleviates letrozole-induced polycystic ovary syndrome in mice by modulating inflammatory mediators and antioxidant signaling pathways. (PubMed, J Reprod Immunol) - "CGA mitigates LZ-induced PCOS through the dual mechanism of modulating the PI3K/AKT-Nrf2 antioxidant defense mechanism and suppression of the NLRP3 inflammasome/NF-κB p65/IL-1β inflammatory pathway. DATA AVAILABILITY: The data supporting the findings of this study are available upon request from the corresponding author."

Journal • Preclinical • Diabetes • Endocrine Disorders • Inflammation • Polycystic Ovary Syndrome • IL1B • NLRP3

Noncompetitive inhibition of sodium-glucose cotransporter by unripe apple polyphenol extract in the mouse small intestine. (PubMed, Biomed Res) - "In this study, we examined the inhibitory effects of an unripe apple polyphenol extract, ApplePhenon® (AP), and its constituents-including Pz, phloretin, procyanidins B1 and B2, chlorogenic acid, gallic acid, catechin, epicatechin, epigallocatechin gallate, and quercetin-on SGLTs using the Ussing chamber in mouse small intestinal mucosa...Although Pz is known to inhibit SGLT1 competitively, AP and its constituents, including Pz, phloretin, and quercetin, showed noncompetitive inhibition in this study. These findings warrant further investigation into the mechanisms underlying SGLT inhibition by AP."

Journal • Preclinical

Chlorogenic acid alleviates lipopolysaccharide-induced cognitive dysfunction through inhibiting CCR7-mediated neuroinflammation. (PubMed, J Neuroimmunol) - "Importantly, we demonstrated CGA ameliorated LPS-induced cognitive dysfunction, at least in part, through inhibition of CCR7-mediated astrocyte activation and its downstream NF-κB, p38 and JNK pathway activation. Collectively, precise elucidation of the inhibitory effect of CGA on CCR7 signaling in LPS-stimulated mice contributes to the development of strategies for controlling neuroinflammation-mediated cognitive disorders."

IO biomarker • Journal • CNS Disorders • Cognitive Disorders • Developmental Disorders • Inflammation • BCL2 • CCR7 • DLG4 • IL1B • IL6 • TNFA