glycafilin (PA 04-001) / ProtAffin - LARVOL DELTA

Designing a CXCL8-hsa chimera as potential immunmodulator of the tumor micro-environment. (PubMed, Front Immunol) - "We have previously engineered a CXCL8-based dominant-negative decoy ("PA401") with strongly increased GAG binding affinity combined with complete GPCR knockout, which was originally developed for the treatment of COPD. When combined with Keytruda (Pembrolizumab), a PD-1 inhibitor, it enhances some of its known activities, indicating potential synergistic effects, but further investigation is needed due to the observed increase in soluble IL-6 and limitations in dose selection for future in vivo studies. By prolonging the presence of engineered chemokine mutants in the bloodstream and optimizing their stability, these strategies aim to enhance the therapeutic efficacy of CXCL8-based interventions, offering promising avenues for the treatment of several CXCL8-mediated pathologies, including cancer."

Biomarker • IO biomarker • Journal • Chronic Obstructive Pulmonary Disease • Immunology • Infectious Disease • Oncology • Pulmonary Disease • Respiratory Diseases • CXCL8 • IL6

Enterocin Cross-Resistance Mediated by ABC Transport Systems. (PubMed, Microorganisms) - "To this end, we designed different mutants (Tn5 derivative and deletion mutants) of the as-48 gene cluster in Enterococcus faecalis and cloned them into the pAM401 shuttle vector. Antimicrobial activity assays showed that enterocin AS-48 Transporter-2 is responsible for cross-resistance between AS-48 and MR10A/B enterocin producers and allowed identification of the MR10A/B immunity gene system. These findings open the way to the investigation of resistance beyond homologous bacteriocins."

Journal

Depletion of BAFF cytokine exacerbates infection in Pseudomonas aeruginosa infected mice. (PubMed, J Cyst Fibros) - "Overall, our data demonstrate for the first time that BAFF is an important regulatory molecule helping to maintain the immunological response to infection and clearance of lung infection."

Journal • Preclinical • Cystic Fibrosis • Fibrosis • Immunology • Respiratory Diseases

Intracellular Accumulation of Linezolid and Florfenicol in OptrA-Producing Enterococcus faecalis and Staphylococcus aureus. (PubMed, Molecules) - "...No significant differences were observed in the patterns of drug accumulation among E. faecalis JH2-2, E. faecalis JH2-2/pAM401, and E. faecalis JH2-2/pAM401+optrA, but also among S. aureus RN4220, S. aureus RN4220/pAM401, and S. aureus RN4220/pAM401+optrA. ANOVA scores also suggested similar accumulation conditions of the two target compounds in susceptible strains and optrA-carrying strains. Based on our findings, the mechanism of optrA-mediated resistance to oxazolidinones and phenicols obviously does not involve active efflux and the OptrA protein does not confer resistance via efflux like other ABC transporters."

Journal