janthinobacterium lividum (DBI-001) / DermBiont - LARVOL DELTA

Complete genome sequences of five aquatic Janthinobacterium lividum strains collected in Austria. (PubMed, Microbiol Resour Announc) - "Each encodes a full violacein biosynthesis pathway. Phylogenomic analysis places most strains close to the type strains, with one strain more distantly related."

Journal

Bioconversion of a Dairy By-Product (Scotta) into Mannitol-Stabilized Violacein via Janthinobacterium lividum Fermentation. (PubMed, Microorganisms) - "Overall, this study highlights the potential of scotta as a sustainable fermentation substrate and presents a promising encapsulation approach for violacein stabilization. However, further investigations are needed to optimize the spray-drying process, specifically, to characterize the microgranules and to determine their storage stability."

Journal • Infectious Disease • Oncology

Exploring the application of the microbial pigment violacein as a sustainable probe molecule for recycled paper-based SERS substrates. (PubMed, Front Chem) - "This study reports for the first time the implementation of the microbial dye violacein, obtained by Janthinobacterium lividum batch cultivation, as SERS probe, as a suitable substitutive to the most employed toxic chemical dye Rhodamine 6G (R6G)...The platform showed a remarkable sensitivity, achieving a detection limit of 10-7 M for violacein, allowing to point out the strong potential of this natural microbial dye as a sustainable probe molecule for low-concentration analytes detection on SERS-active substrates, making them suitable for several application fields, such as environmental monitoring, food safety, cultural heritage analysis and diagnostics. This study demonstrates the feasibility of implementing eco-friendly materials in the development of chemical sensors as a sustainable innovation in environmental science by minimizing the ecological impact."

Journal

Impact of inoculation of the sap collection system with three Pseudomonadota strains on maple syrup microbial and organoleptic quality. (PubMed, Can J Microbiol) - "MSB2019, Janthinobacterium lividum 100-P12-9, and Pseudomonas fluorescens ATCC 17926, were inoculated to independent sap collection system throughout two sugaring seasons...While sap yield and primary microbial load remained unaffected, inoculation treatments corresponded to shifts in flavor attributes of the syrup. These findings indicate that inoculating sap collection systems with targeted strains can positively influence maple syrup quality, particularly in enhancing desirable flavor profiles, suggesting promising applications for syrup production."

Journal

Janthinobacterium aestuarii sp. nov., a novel violacein-producing bacterium isolated from the Tidal Basin in Washington, D.C., USA. (PubMed, Int J Syst Evol Microbiol) - "For more precise taxonomic inference, a phylogenomic analysis was conducted and indicated that strain TB1-E2-13T was most closely related to Janthinobacterium lividum, 'Janthinobacterium kumbetense', Janthinobacterium rivuli and Janthinobacterium violaceinigrum...is proposed. The type strain is TB1-E2-13T (=ATCC TSD-339T=JCM 36076T)."

Journal

Extracellular vesicles of Janthinobacterium lividum as violacein carriers in melanoma cell treatment. (PubMed, Appl Microbiol Biotechnol) - "• EVs were characterised for size, morphology and protein composition. • Studies on human keratinocytes and a melanoma cell model confirmed that the activity of violacein applied in the encapsulated form of EVs is similar to that of its organic solvent extract, but their production is much more environmentally friendly."

Journal • Melanoma • Oncology • Solid Tumor

Priority effects alter microbiome composition and increase abundance of probiotic taxa in treefrog tadpoles. (PubMed, Appl Environ Microbiol) - "Second, we experimentally inoculated embryos to determine if priority effects (i) could be used to increase the relative abundance of Janthinobacterium lividum, an amphibian-associated bacteria capable of preventing fungal infection, and (ii) would lead to observed differences in the relative abundances of two closely related bacteria from the genus Pseudomonas...Further, we provide support for the use of bacterial priority effects as a mechanism to increase the relative abundance of target probiotic taxa in a developing host. While our results show that priority effects are not universally effective across all host-associated bacteria, our ability to increase the relative abundance of specific probiotic taxa may enhance conservation strategies that rely on captive rearing of endangered vertebrates."

Journal • Infectious Disease

Improvement of violacein production using abiotic stresses and microbial adaptation. (PubMed, World J Microbiol Biotechnol) - "The aim of the current research was to improve violacein production with Janthinobacterium lividum using abiotic stresses and bacterial adaptation against stress...Then, the production of violacein under hydrogen peroxide (H2O2) and ampicillin (Amp) stresses and acyl homoserine lactone (AHL) was evaluated...Also, the expression of vioA increased in the presence of Amp but H2O2 had no significant effect on vioA expression. Totally, we showed that microbial adaptation and abiotic stresses are cost-effective methods to generate significant improvement in violacein production."

Journal

A Trial to Evaluate the Safety and Efficacy of DBI-001 Gel, DBI-002 Gel, and Aqueous Gel in Subjects With Tinea Pedis (clinicaltrials.gov) - P2 | N=24 | Active, not recruiting | Sponsor: DermBiont, Inc. | Trial completion date: Dec 2022 ➔ Dec 2023 | Trial primary completion date: Oct 2022 ➔ Dec 2023

Trial completion date • Trial primary completion date • Dermatology

A Trial to Evaluate the Safety and Efficacy of DBI-001 Gel, DBI-002 Gel, and Aqueous Gel in Subjects With Onychomycosis (clinicaltrials.gov) - P2 | N=60 | Suspended | Sponsor: DermBiont, Inc. | Initiation date: Oct 2022 ➔ Mar 2023

Trial initiation date • Infectious Disease

Together or Alone: Evaluating the Pathogen Inhibition Potential of Bacterial Cocktails against an Amphibian Pathogen. (PubMed, Microbiol Spectr) - "Several experimental trials have tested the use of Janthinobacterium lividum to reduce mortality due to Bd infections, usually in single-strain amendments...For Colorado boreal toads, we addressed whether there are other bacterial strains that also inhibit Bd (potentially better than does J. lividum) and whether we can create two-strain probiotics that function better than do single-strain probiotics. In addition, we evaluate which types of interactions occur between two-strain combinations and what these results mean in the context of adding a probiotic to an existing amphibian skin microbiome."

Journal • Dermatology • Infectious Disease

Janthinobacterium sp. Strain SLB01 as Pathogenic Bacteria for Sponge Lubomirskia baikalensis. (PubMed, Pathogens) - "with the described strain of Janthinobacterium lividum MTR. This study will help expand our understanding of microbial interactions and determine one of the causes in the development of diseases and death in Baikal sponges."

Journal • Developmental Disorders

Study the Safety and Efficacy of DBI-001 Gel Versus Aqueous Gel (clinicaltrials.gov) - P2 | N=10 | Terminated | Sponsor: DermBiont, Inc. | N=20 ➔ 10 | Suspended ➔ Terminated; Sponsor suspension and not due to safety or site-related matters.

Enrollment change • Trial termination • Atopic Dermatitis • Dermatitis • Dermatology • Immunology

A Trial to Evaluate the Safety and Efficacy of DBI-001 Gel, DBI-002 Gel, and Aqueous Gel in Subjects With Tinea Pedis (clinicaltrials.gov) - P2 | N=24 | Active, not recruiting | Sponsor: DermBiont, Inc. | Recruiting ➔ Active, not recruiting

Enrollment closed • Dermatology

A Trial to Evaluate the Safety and Efficacy of DBI-001 Gel, DBI-002 Gel, and Aqueous Gel in Subjects With Onychomycosis (clinicaltrials.gov) - P2 | N=60 | Suspended | Sponsor: DermBiont, Inc. | Recruiting ➔ Suspended

Trial suspension • Infectious Disease

Study the Safety and Efficacy of DBI-001 Gel Versus Aqueous Gel (clinicaltrials.gov) - P2 | N=20 | Suspended | Sponsor: DermBiont, Inc. | Recruiting ➔ Suspended

Trial suspension • Atopic Dermatitis • Dermatitis • Dermatology • Immunology

Alhagi maurorum extract modulates quorum sensing genes and biofilm formation in Proteus mirabilis. (PubMed, Sci Rep) - "The AME's effect on adhesion and quorum sensing (QS) were investigated by in vitro adhesion assay on cell culture and agar overlay assay using Janthinobacterium lividum (ATCC 12472) as a biosensor strain...This study showed that AME as a natural compound reduced biofilm formation of P. mirabilis by targeting virulence factor genes, quorum sensing, and other strategies that include preventing the adhesion of P. mirabilis to the cells. The results suggest that A. maurorum extract might have the potential to be considered for preventing UTIs caused by P. mirabilis."

Journal • Addiction (Opioid and Alcohol) • Infectious Disease • Nephrology

A Trial to Evaluate the Safety and Efficacy of DBI-001 Gel, DBI-002 Gel, and Aqueous Gel in Subjects With Tinea Pedis (clinicaltrials.gov) - P2 | N=24 | Recruiting | Sponsor: DermBiont, Inc.

New P2 trial • Dermatology

A Trial to Evaluate the Safety and Efficacy of DBI-001 Gel, DBI-002 Gel, and Aqueous Gel in Subjects With Onychomycosis (clinicaltrials.gov) - P2 | N=60 | Recruiting | Sponsor: DermBiont, Inc.

New P2 trial • Infectious Disease

Environmental factors affecting the diversity of psychrophilic microbial community in the high altitude snow-fed lake Hemkund, India. (PubMed, Curr Res Microb Sci) - "Janthinobacterium lividum, Pseudomonas tolaasii, Pseudomonas rhodesiae, and Pseudomonas fluorescens are a few important and key species that were found in the lake water. The present study on the diversity of psychrophilic microorganisms in the high altitude Lake Hemkund could be a great reference for further research activities on comparable viewpoints in different parts of the Himalaya. This baseline information can also help the administrative officials to take necessary steps for its conservation and management."

Journal

Study the Safety and Efficacy of DBI-001 Gel Versus Aqueous Gel (clinicaltrials.gov) - P2 | N=20 | Recruiting | Sponsor: DermBiont, Inc. | Trial primary completion date: Jun 2022 ➔ Oct 2022

Trial primary completion date • Atopic Dermatitis • Dermatitis • Dermatology • Immunology

Violacein and its antifungal activity: comments and potentialities. (PubMed, Lett Appl Microbiol) - "Violacein is an important natural antimicrobial pigment that is mainly produced by Chromobacterium violaceum and Janthinobacterium lividum. It presents a significant range of effects against phytopathogenic and human fungi, besides being featured as having low toxicity, and by its important ecological role in protecting amphibian species and applications in dyed medical fabric. The hypothesis about violacein's action mechanisms against mucormycosis (Rhizopus arrhizus) and candidiasis (Candida auris) is herein discussed based on data available in the scientific literature."

Journal • Review • Candidiasis • Infectious Disease

Study the Safety and Efficacy of DBI-001 Gel Versus Aqueous Gel (clinicaltrials.gov) - P2 | N=20 | Recruiting | Sponsor: DermBiont, Inc. | Trial primary completion date: Mar 2022 ➔ Jun 2022

Trial primary completion date • Atopic Dermatitis • Dermatitis • Dermatology • Immunology

Study the Safety and Efficacy of DBI-001 Gel Versus Aqueous Gel (clinicaltrials.gov) - P2 | N=20 | Recruiting | Sponsor: DermBiont, Inc.

New P2 trial • Atopic Dermatitis • Dermatitis • Dermatology • Immunology

Effectiveness of antifungal treatments during chytridiomycosis epizootics in populations of an endangered frog. (PubMed, PeerJ) - "Experiments included treatment of early life stages (tadpoles and subadults) with the antifungal drug itraconazole, treatment of adults with itraconazole, and augmentation of the skin microbiome of subadults with Janthinobacterium lividum, a commensal bacterium with antifungal properties. Collectively, these results indicate that our mitigation efforts were ineffective in causing long-term changes in frog-Bd dynamics and increasing population persistence, due largely to the inability of early life stages to mount an effective immune response against Bd. This results in repeated recruitment failure and a low probability of population persistence in the face of ongoing Bd infection."

Clinical • Journal • Infectious Disease