Nrf2 alleviates acute ischemic stroke induced ferroptosis via regulating xCT/GPX4 pathway. (PubMed, Free Radic Biol Med) - Mar 1, 2025 - "In vitro experiments, we investigated the mechanism of action of Nrf2 on the establishment of a ferroptosis cell model induced by Erastin by overexpressing or silencing Nrf2 expression using shRNA in SH-SY5Y cells.Ferroptosis played an important role in AIS, and Fer-1 inhibited iron accumulation and alleviated neuronal damage caused by AIS.Oltipraz attenuated AIS-induced neuronal damage and cerebral infarction by increasing cortical blood flow (CBF). Furthermore, both ML385 treatment and Nrf2 knockout mice exacerbated oxidative stress injury and iron overload and downregulated the expression of both xCT and GPX4. Consistent with the in vivo results, Nrf2 conferred ferroptosis resistance in vitro upon exposure to compounds that induce ferroptosis, by modulating the xCT/GPX4 pathway.The present study confirmed that Nrf2 could attenuate AIS-induced neuronal ferroptosis and oxidative stress by regulating xCT/GPX4."
Journal • Cardiovascular • CNS Disorders • Hematological Disorders • Ischemic stroke • GPX4 • NFE2L2 • SLC7A11
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Chengyin Wang; He Zhao; Lingling Qiu; Qing Liu; Ting Zeng; Weifeng Zhu; Xuexin Xie; Yiming Bi; Yujun Ye
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