CFTR Assembles into Multiple Protein Complexes in the Kidney That Regulate Proliferation and Protein Trafficking to the Cilia in ADPKD (KIDNEY WEEK 2026) - Oct 2, 2026 - Abstract #SA-PO0137; Pres time: Oct 24, 2026; 10:00 AM - 12:00 PM; Location: Exhibit Hall A, Convention Center; "Methods We used immunoprecipitation and proximity ligase assays (PLA) combined with immunofluorescence experiments in pkd1 R3277/ R3277C (RC) mutant mice and cell lines treated with the CFTR modulators such as VX-809...Finally, we found that the interactions of CFTR with scaffold proteins NHERF 1 & 2 are also abnormal in ADPKD suggesting that CFTR's interaction with TAZ and YAP mostly involves the NHERF proteins via CFTR's PDZ binding domain. Conclusion We provide here an understanding of how CFTR via complex interactions with multiple pathways through molecular interactions can affect proliferation and protein trafficking to the primary cilium highlighting its function both as a chloride channel and conductance regulator and providing an understanding of how CFTR modulators can be therapeutic."
Autosomal Dominant Polycystic Kidney Disease • Genetic Disorders • Nephrology • Polycystic Kidney Disease • Renal Disease • PKD1 • PRKD1 • SEPTIN2
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Liudmila Cebotaru; Abhishek Sharma; Masoud Afshani; Tanvi Sharma; William B. Guggino
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